PMID- 19947251
OWN - NLM
STAT- MEDLINE
DCOM- 20110623
LR  - 20151119
IS  - 2096-7993 (Print)
IS  - 2096-7993 (Linking)
VI  - 23
IP  - 15
DP  - 2009 Aug
TI  - [Effects of celecoxib on the proliferation and apoptosis of human nasopharyngeal 
      carcinoma cell line CNE-2].
PG  - 682-5
AB  - OBJECTIVE: To detect the effect of Celecoxib on the proliferation and apoptosis 
      of human nasopharyngeal carcinoma cell line CNE-2. METHOD: The growth inhibition 
      rate of CNE-2 by Celecoxib was evaluated with MTT method. Apoptosis related 
      morphology changes were observed with transmission electron microscopy (TEM). The 
      cell cycle and apoptosis were measured with flow cytometric method (FCM). 
      Apoptotic index (AI) was counted by the TDT-mediated dUTP-biotin nick 
      end-labeling (TUNEL) assay. RESULT: The growth of CNE-2 cell was inhibited by 
      celecoxib in a dose-and time-dependent manner. Apoptosis with nuclear chromatin 
      condensation, cell shrinkage, periplasm loss and the formation of apoptotic 
      bodies was observed with TEM. Apoptotic rates of CNE-2 cells treated with 80 and 
      100 micromol/L celecoxib were (10.47+/-0.18)% and (20.17+/-0.55)% respectively, 
      significantly higher than those of the control group (1.57+/-0.27)% with FCM. The 
      percentage of G0/G1 phase cells increased, whereas the S and G2/M phases cells 
      decreased in a dose-dependent manner after the treatment. TUNEL assay showed that 
      the apoptosis ratio (AI) of CNE-2 treated with Celecoxib was higher than control 
      group (P<0.01). CONCLUSION: Celecoxib can inhibit the growth of human 
      nasopharyngeal carcinoma cell line CNE-2 and induce the cell apoptosis, which may 
      be related to blocking the cell cycle progress of CNE-2 cells.
FAU - Xu, Xinhua
AU  - Xu X
AD  - Department of Oncology, Central Hospital of Yichang City, the First Clinical 
      Medical College of Three Gorges University, Yichang, 443003, China. 
      xuxinhua@medmail.com.cn
FAU - Yi, Fang
AU  - Yi F
FAU - Fu, Xiangyang
AU  - Fu X
FAU - Li, Daojun
AU  - Li D
FAU - Huang, Qiao
AU  - Huang Q
FAU - Du, Jingtao
AU  - Du J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical 
      otorhinolaryngology, head, and neck surgery
JID - 101303164
RN  - 0 (Cyclooxygenase 2 Inhibitors)
RN  - 0 (Pyrazoles)
RN  - 0 (Sulfonamides)
RN  - JCX84Q7J1L (Celecoxib)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Celecoxib
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Cyclooxygenase 2 Inhibitors/*pharmacology
MH  - Humans
MH  - Nasopharyngeal Neoplasms/pathology
MH  - Pyrazoles/*pharmacology
MH  - Sulfonamides/*pharmacology
EDAT- 2009/12/02 06:00
MHDA- 2011/06/24 06:00
CRDT- 2009/12/02 06:00
PHST- 2009/12/02 06:00 [entrez]
PHST- 2009/12/02 06:00 [pubmed]
PHST- 2011/06/24 06:00 [medline]
PST - ppublish
SO  - Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2009 Aug;23(15):682-5.