PMID- 19947479
OWN - NLM
STAT- MEDLINE
DCOM- 20111215
LR  - 20091201
IS  - 1001-5515 (Print)
IS  - 1001-5515 (Linking)
VI  - 26
IP  - 5
DP  - 2009 Oct
TI  - [Studies on the growth of rabbit skin fibroblasts on the surfaces of acellular 
      dermal matrix].
PG  - 1010-5
AB  - The growth of fibroblasts on the acellular dermal matrix (ADM) was studied. The 
      fibroblasts isolated from the skin of an adult New Zealand Rabbit were cultured 
      in vitro and identified subsequently. After the cells were inoculated on the ADM 
      as seeds, the adhesion rate and the growth ability were examined, and cellular 
      morphology was assayed with DAPI fluorescent staining and Scanning electron 
      microscope (SEM). The possibilities of applying ADM as cells carrier or deliverer 
      in the field of transplantation were evaluated. The result revealed that pure 
      fibroblasts were isolated through the specific method. Skin fibroblasts could 
      adhere to ADM easily, and the adhesion rate was 96.78%, displaying no significant 
      difference (P > 0.05) when compared with that rate of the control holes. The 
      cells on the scaffolds and those on the control holes showed similar growth 
      tendencies, but the activity of the former was lower (P < 0.01). The integral 
      nucleus with blue fluorescence could be observed on the ADM under fluorescence 
      microscope. The number of fibroblasts scaled up with the cultured time, The 
      results of SEM showed that the state of cell was good and the fibroblasts were 
      fused into a layer after being cultured for 5-10d. So rabbit fibroblasts can 
      attach, survive, grow and proliferate on the ADM in a healthy way. It is entirely 
      possible to use ADM as an appropriate scaffold material for the culture of 
      fibroblasts and as a material for transplantations.
FAU - Guan, Linbo
AU  - Guan L
AD  - The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, 
      Sichuan University, Chengdu 610065, China.
FAU - Dan, Weihua
AU  - Dan W
FAU - Lin, Hai
AU  - Lin H
FAU - Dan, Nianhua
AU  - Dan N
FAU - Wang, Kunyu
AU  - Wang K
FAU - Liao, Longli
AU  - Liao L
FAU - Li, Zhiqiang
AU  - Li Z
FAU - Chen, Min
AU  - Chen M
FAU - Zeng, Rui
AU  - Zeng R
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
JT  - Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = 
      Shengwu yixue gongchengxue zazhi
JID - 9426398
RN  - 0 (Biocompatible Materials)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials
MH  - Cell Adhesion
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Dermis/*cytology
MH  - Fibroblasts/*cytology
MH  - Rabbits
MH  - Skin/*cytology
MH  - *Skin, Artificial
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds
EDAT- 2009/12/02 06:00
MHDA- 2011/12/16 06:00
CRDT- 2009/12/02 06:00
PHST- 2009/12/02 06:00 [entrez]
PHST- 2009/12/02 06:00 [pubmed]
PHST- 2011/12/16 06:00 [medline]
PST - ppublish
SO  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2009 Oct;26(5):1010-5.