PMID- 19948265
OWN - NLM
STAT- MEDLINE
DCOM- 20100223
LR  - 20131121
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 28
IP  - 5
DP  - 2010 Feb 3
TI  - Adjuvant effects of chicken interleukin-18 in avian Newcastle disease vaccine.
PG  - 1148-55
LID - 10.1016/j.vaccine.2009.11.042 [doi]
AB  - Interleukin-18 (IL-18) can induce interferon-gamma (IFN-gamma) production and 
      promote Th1 immunity, and hence, it modulates immune functions. In the present 
      study, the in vitro and in vivo immunomodulatory activities of full length or 
      mature chicken IL-18 expressed in a prokaryotic expression system (pCHIL18-F and 
      pCHIL18-M, respectively) and chicken IL-18 expressed in a eukaryotic expression 
      system (euCHIL18) were examined. Results showed that pCHIL18-F, pCHIL18-M and 
      euCHIL18 significantly enhanced IFN-gamma mRNA expression in chicken splenocytes, 
      which successfully increased IFN-gamma-induced nitric oxide (NO) synthesis by 
      macrophages. Vaccination with cell-cultured Newcastle disease vaccine (NDTC) 
      co-administrated with pCHIL18-F, pCHIL18-M or euCHIL18 resulted in significant 
      increments of hemagglutination inhibition (HI) titers, cell proliferation of 
      peripheral blood mononuclear cells (PBMC), and ratios of CD8(+) to CD4(+) in 
      chickens compared with inoculation of PBS or NDTC alone. Thus, full length and 
      mature chicken IL-18 expressed using a prokaryotic system and using a eukaryotic 
      system showed equivalent in vitro and in vivo biological activities, and all 
      forms effectively enhanced cell-mediated and humoral immunity, suggesting 
      possible future use as a potential adjuvant in chicken NDTC vaccine production.
CI  - (c) 2009 Elsevier Ltd. All rights reserved.
FAU - Hung, Li-Hsiang
AU  - Hung LH
AD  - Department of Veterinary Medicine, National Pingtung University of Science & 
      Technology, 1, Hseuh Fu Road, Neipu, Pingtung 912, Taiwan, ROC.
FAU - Li, Hsin-Pei
AU  - Li HP
FAU - Lien, Yi-Yang
AU  - Lien YY
FAU - Wu, Mei-Li
AU  - Wu ML
FAU - Chaung, Hso-Chi
AU  - Chaung HC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091128
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Interleukin-18)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Vaccines)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adjuvants, Immunologic/genetics/*pharmacology
MH  - Animals
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Chickens
MH  - Immunity, Cellular/drug effects/immunology
MH  - Immunity, Humoral/drug effects/immunology
MH  - Interferon-gamma/immunology
MH  - Interleukin-18/genetics/immunology/*pharmacology
MH  - Newcastle Disease/immunology/*prevention & control
MH  - Newcastle disease virus/*immunology
MH  - Nitric Oxide/immunology
MH  - Recombinant Proteins/genetics/immunology/pharmacology
MH  - Th1 Cells/immunology
MH  - Viral Vaccines/genetics/immunology/*pharmacology
EDAT- 2009/12/02 06:00
MHDA- 2010/02/24 06:00
CRDT- 2009/12/02 06:00
PHST- 2009/07/23 00:00 [received]
PHST- 2009/10/21 00:00 [revised]
PHST- 2009/11/11 00:00 [accepted]
PHST- 2009/12/02 06:00 [entrez]
PHST- 2009/12/02 06:00 [pubmed]
PHST- 2010/02/24 06:00 [medline]
AID - S0264-410X(09)01820-9 [pii]
AID - 10.1016/j.vaccine.2009.11.042 [doi]
PST - ppublish
SO  - Vaccine. 2010 Feb 3;28(5):1148-55. doi: 10.1016/j.vaccine.2009.11.042. Epub 2009 
      Nov 28.