PMID- 19949909
OWN - NLM
STAT- MEDLINE
DCOM- 20100928
LR  - 20240317
IS  - 1466-1268 (Electronic)
IS  - 1355-8145 (Print)
IS  - 1355-8145 (Linking)
VI  - 15
IP  - 4
DP  - 2010 Jul
TI  - Hyperbaric oxygen induces a cytoprotective and angiogenic response in human 
      microvascular endothelial cells.
PG  - 431-42
LID - 10.1007/s12192-009-0159-0 [doi]
AB  - A genome-wide microarray analysis of gene expression was carried out on human 
      microvascular endothelial cells (HMEC-1) exposed to hyperbaric oxygen treatment 
      (HBOT) under conditions that approximated clinical settings. Highly up-regulated 
      genes included immediate early transcription factors (FOS, FOSB, and JUNB) and 
      metallothioneins. Six molecular chaperones were also up-regulated immediately 
      following HBOT, and all of these have been implicated in protein damage control. 
      Pathway analysis programs identified the Nrf-2-mediated oxidative stress response 
      as one of the primary responders to HBOT. Several of the microarray changes in 
      the Nrf2 pathway and a molecular chaperone were validated using quantitative PCR. 
      For all of the genes tested (Nrf2, HMOX1, HSPA1A, M1A, ACTC1, and FOS), HBOT 
      elicited large responses, whereas changes were minimal following treatment with 
      100% O(2) in the absence of elevated pressure. The increased expression of 
      immediate early and cytoprotective genes corresponded with an HBOT-induced 
      increase in cell proliferation and oxidative stress resistance. In addition, HBOT 
      treatment enhanced endothelial tube formation on Matrigel plates, with 
      particularly dramatic effects observed following two daily HBO treatments. 
      Understanding how HBOT influences gene expression changes in endothelial cells 
      may be beneficial for improving current HBOT-based wound-healing protocols. These 
      data also point to other potential HBOT applications where stimulating protection 
      and repair of the endothelium would be beneficial, such as patient 
      preconditioning prior to major surgery.
FAU - Godman, Cassandra A
AU  - Godman CA
AD  - Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, 
      USA. Cassandra.Godman@uconn.edu
FAU - Chheda, Kousanee P
AU  - Chheda KP
FAU - Hightower, Lawrence E
AU  - Hightower LE
FAU - Perdrizet, George
AU  - Perdrizet G
FAU - Shin, Dong-Guk
AU  - Shin DG
FAU - Giardina, Charles
AU  - Giardina C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091201
PL  - Netherlands
TA  - Cell Stress Chaperones
JT  - Cell stress & chaperones
JID - 9610925
RN  - 0 (Molecular Chaperones)
RN  - 0 (NF-E2-Related Factor 2)
SB  - IM
MH  - Cytoprotection
MH  - Endothelial Cells/cytology/*metabolism
MH  - Endothelium, Vascular/*cytology
MH  - Gene Expression Regulation
MH  - Humans
MH  - *Hyperbaric Oxygenation
MH  - Microarray Analysis
MH  - Molecular Chaperones/genetics/metabolism
MH  - NF-E2-Related Factor 2/genetics/metabolism
MH  - Neovascularization, Physiologic
MH  - Signal Transduction
MH  - Up-Regulation
PMC - PMC3082642
EDAT- 2009/12/02 06:00
MHDA- 2010/09/30 06:00
PMCR- 2011/01/01
CRDT- 2009/12/02 06:00
PHST- 2009/09/17 00:00 [received]
PHST- 2009/11/04 00:00 [accepted]
PHST- 2009/11/02 00:00 [revised]
PHST- 2009/12/02 06:00 [entrez]
PHST- 2009/12/02 06:00 [pubmed]
PHST- 2010/09/30 06:00 [medline]
PHST- 2011/01/01 00:00 [pmc-release]
AID - S1355-8145(23)00568-0 [pii]
AID - 159 [pii]
AID - 10.1007/s12192-009-0159-0 [doi]
PST - ppublish
SO  - Cell Stress Chaperones. 2010 Jul;15(4):431-42. doi: 10.1007/s12192-009-0159-0. 
      Epub 2009 Dec 1.