PMID- 19950368
OWN - NLM
STAT- MEDLINE
DCOM- 20100216
LR  - 20220311
IS  - 1531-4995 (Electronic)
IS  - 0023-852X (Linking)
VI  - 120
IP  - 2
DP  - 2010 Feb
TI  - Targeted imaging modality selection for bacterial biofilms in chronic 
      rhinosinusitis.
PG  - 427-31
LID - 10.1002/lary.20705 [doi]
AB  - OBJECTIVES/HYPOTHESIS: Biofilms are increasingly recognized as having an 
      etiological role in chronic rhinosinusitis (CRS). Research into biofilms in CRS 
      currently relies on microscopic imaging techniques, none of which are universally 
      accepted. This study compares LIVE/DEAD BacLight (Invitrogen Corp., Carlsbad, CA) 
      staining and fluorescence in situ hybridization (FISH), both utilizing the 
      confocal scanning laser microscope (CSLM) for biofilm determination and 
      characterization in CRS patients. STUDY DESIGN: Prospective study. METHODS: 
      Twenty CRS patients undergoing endoscopic sinus surgery were recruited for the 
      study. Sinus mucosal tissue harvested at the time of surgery underwent both the 
      BacLight/CSLM and FISH/CSLM protocols for biofilm determination and 
      characterization. RESULTS: Combining the results of both protocols, 18/20 (90%) 
      patients had bacterial biofilms demonstrable on at least one modality. The high 
      biofilm detection rate combining the two techniques suggests the prevalence of 
      biofilms in CRS may be greater than previously reported. The protocols had 
      equivalent results in 15/20 patients. Using the differences observed in the 
      remaining five patients, we can highlight the most appropriate use for each 
      technique. CONCLUSIONS: BacLight/CSLM and FISH/CSLM are complementary techniques 
      for biofilm determination and characterization. Both protocols are suited to 
      different research areas and the selection of technique used should be based on 
      the specific objectives of the research protocol. In this way we can utilize the 
      advantages of each technique to facilitate effective research.
FAU - Foreman, Andrew
AU  - Foreman A
AD  - Department of Surgery-Otorhinolaryngology, Head and Neck Surgery, University of 
      Adelaide and Flinders University, Adelaide, Australia.
FAU - Singhal, Deepti
AU  - Singhal D
FAU - Psaltis, Alkis J
AU  - Psaltis AJ
FAU - Wormald, Peter-John
AU  - Wormald PJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Laryngoscope
JT  - The Laryngoscope
JID - 8607378
RN  - 0 (Fluorescent Dyes)
SB  - IM
CIN - Laryngoscope. 2011 Sep;121(9):2043-4; author reply 2045-6. PMID: 22024862
MH  - Bacteria/*isolation & purification
MH  - *Biofilms
MH  - Chronic Disease
MH  - Female
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Microscopy, Confocal
MH  - Microscopy, Fluorescence
MH  - Middle Aged
MH  - Nasal Mucosa/*microbiology
MH  - Paranasal Sinuses/*microbiology/surgery
MH  - Rhinitis/*microbiology
MH  - Sinusitis/*microbiology
EDAT- 2009/12/02 06:00
MHDA- 2010/02/17 06:00
CRDT- 2009/12/02 06:00
PHST- 2009/12/02 06:00 [entrez]
PHST- 2009/12/02 06:00 [pubmed]
PHST- 2010/02/17 06:00 [medline]
AID - 10.1002/lary.20705 [doi]
PST - ppublish
SO  - Laryngoscope. 2010 Feb;120(2):427-31. doi: 10.1002/lary.20705.