PMID- 19951089
OWN - NLM
STAT- MEDLINE
DCOM- 20100702
LR  - 20181201
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Linking)
VI  - 16
IP  - 4
DP  - 2010 Apr
TI  - Multilineage potential of bone-marrow-derived mesenchymal stem cell cell sheets: 
      implications for tissue engineering.
PG  - 1421-31
LID - 10.1089/ten.TEA.2009.0501 [doi]
AB  - Bone-marrow-derived mesenchymal stem cells (BMSCs) are a promising source of 
      cells for tissue engineering due to their multilineage mesenchymal 
      differentiation potential. Their ability to proliferate and differentiate into 
      the osteogenic, chondrogenic, and adipogenic lineage makes them an attractive 
      cell source as compared to the terminally differentiated cells. In tissue 
      engineering, use of cell sheet technology is gaining popularity. It is based on 
      culturing cells until hyperconfluence, and it has resulted in the reduction of 
      the number of cells lost when seeding onto scaffolds. Thus, formation of cell 
      sheets with multipotent cells, such as BMSCs, would be a promising alternative to 
      the conventional method of cell seeding, that is, single-cell suspension. 
      However, the multilineage potential of BMSC cell sheets has yet to be verified. 
      Therefore, the aim of this study was to characterize the formation of a 
      hyperconfluent BMSC cell sheet as well as the effects of the hyperconfluent 
      culture conditions on the multipotentiality of BMSCs. Our results showed that the 
      BMSC cell sheets remained viable. The cell sheets were rich with type I collagen 
      and were shown to have retained their multipotentiality. Hence, the use of BMSC 
      cell sheets for tissue engineering application seems promising.
FAU - See, Eugene Yong-Shun
AU  - See EY
AD  - National University of Singapore, Singapore, Singapore.
FAU - Toh, Siew Lok
AU  - Toh SL
FAU - Goh, James Cho Hong
AU  - Goh JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (DNA Primers)
RN  - 0 (PPAR gamma)
RN  - 0 (RNA, Messenger)
RN  - 0 (SOX9 Transcription Factor)
SB  - IM
MH  - Adipogenesis/genetics
MH  - Animals
MH  - Base Sequence
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Chondrogenesis/genetics
MH  - Coated Materials, Biocompatible
MH  - Core Binding Factor Alpha 1 Subunit/genetics
MH  - DNA Primers/genetics
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Multipotent Stem Cells/*cytology/metabolism
MH  - Osteogenesis/genetics
MH  - PPAR gamma/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Rabbits
MH  - SOX9 Transcription Factor/genetics
MH  - Tissue Engineering/*methods
EDAT- 2009/12/03 06:00
MHDA- 2010/07/03 06:00
CRDT- 2009/12/03 06:00
PHST- 2009/12/03 06:00 [entrez]
PHST- 2009/12/03 06:00 [pubmed]
PHST- 2010/07/03 06:00 [medline]
AID - 10.1089/ten.TEA.2009.0501 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2010 Apr;16(4):1421-31. doi: 10.1089/ten.TEA.2009.0501.