PMID- 19951702 OWN - NLM STAT- MEDLINE DCOM- 20100310 LR - 20161125 IS - 1873-2968 (Electronic) IS - 0006-2952 (Linking) VI - 79 IP - 8 DP - 2010 Apr 15 TI - Polycyclic aromatic hydrocarbon metabolizing cytochrome P450s in freshly prepared uncultured rat blood lymphocytes. PG - 1182-8 LID - 10.1016/j.bcp.2009.11.021 [doi] AB - In an attempt to develop blood lymphocyte cytochrome P450 expression profile as a surrogate to monitor tissue enzyme, the present study aimed to identify the expression and regulation of polycyclic aromatic hydrocarbons (PAHs) responsive CYPs in freshly prepared rat blood lymphocytes. Semi-quantitative and RT-PCR studies demonstrated constitutive and inducible mRNA expression of CYP1A1, 1A2, 1B1 isoenzymes and the associated transcription factors, aryl hydrocarbon receptor (AhR) and AhR translocator (ARNT) in blood lymphocytes. Absolute quantification using RT-PCR revealed several fold lower basal expression of CYP1A1, 1A2 and 1B1 in lymphocytes when compared to the liver. However, significant increase in the mRNA expression of these isoenzymes as well as AhR and ARNT in lymphocytes following pretreatment with 3-methylcholanthrene (MC) have demonstrated that responsiveness is retained in the blood lymphocytes, though the magnitude of increase is several fold lower when compared to liver. This increase in the mRNA expression was found to be associated with an increase in the protein expression of CYP1A1 and 1A2 in blood lymphocytes. Further, CYPs expressed in blood lymphocytes catalysed the O-dealkylation of 7-ethoxy- and 7-methoxyresorufins (ER or MR), though the reactivity was several fold lower in lymphocytes when compared to the liver enzyme. Our data providing quantitative evidence for similarities in the regulation of PAH-regulated CYP in uncultured and non-mitogen stimulated blood lymphocytes with the liver enzyme has led us to suggest that blood lymphocytes could be used as a surrogate to monitor tissue expression of CYPs. CI - 2009 Elsevier Inc. All rights reserved. FAU - Saurabh, Kumar AU - Saurabh K AD - Indian Institute of Toxicology Research, Lucknow, U.P., India. FAU - Sharma, Amit AU - Sharma A FAU - Yadav, Sanjay AU - Yadav S FAU - Parmar, Devendra AU - Parmar D LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20091129 PL - England TA - Biochem Pharmacol JT - Biochemical pharmacology JID - 0101032 RN - 0 (ARNT protein, rat) RN - 0 (Cytochromes) RN - 0 (Polycyclic Aromatic Hydrocarbons) RN - 0 (RNA, Messenger) RN - 0 (Receptors, Aryl Hydrocarbon) RN - 138391-32-9 (Aryl Hydrocarbon Receptor Nuclear Translocator) RN - EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases) RN - EC 1.14.14.1 (Cyp1a2 protein, rat) RN - EC 1.14.14.1 (Cyp1b1 protein, rat) RN - EC 1.14.14.1 (Cytochrome P-450 CYP1A1) RN - EC 1.14.14.1 (Cytochrome P-450 CYP1A2) RN - EC 1.14.14.1 (Cytochrome P-450 CYP1B1) SB - IM MH - Animals MH - Aryl Hydrocarbon Hydroxylases/*genetics MH - Aryl Hydrocarbon Receptor Nuclear Translocator/genetics MH - Blotting, Western MH - Cytochrome P-450 CYP1A1/antagonists & inhibitors/*genetics MH - Cytochrome P-450 CYP1A2 MH - Cytochrome P-450 CYP1B1 MH - Cytochromes/antagonists & inhibitors/*genetics MH - Lymphocytes/*enzymology MH - Male MH - Polycyclic Aromatic Hydrocarbons/*metabolism MH - RNA, Messenger/analysis MH - Rats MH - Rats, Wistar MH - Receptors, Aryl Hydrocarbon/genetics MH - Reverse Transcriptase Polymerase Chain Reaction EDAT- 2009/12/03 06:00 MHDA- 2010/03/11 06:00 CRDT- 2009/12/03 06:00 PHST- 2009/10/07 00:00 [received] PHST- 2009/11/24 00:00 [revised] PHST- 2009/11/24 00:00 [accepted] PHST- 2009/12/03 06:00 [entrez] PHST- 2009/12/03 06:00 [pubmed] PHST- 2010/03/11 06:00 [medline] AID - S0006-2952(09)01033-8 [pii] AID - 10.1016/j.bcp.2009.11.021 [doi] PST - ppublish SO - Biochem Pharmacol. 2010 Apr 15;79(8):1182-8. doi: 10.1016/j.bcp.2009.11.021. Epub 2009 Nov 29.