PMID- 19951731
OWN - NLM
STAT- MEDLINE
DCOM- 20100615
LR  - 20211020
IS  - 1873-4995 (Electronic)
IS  - 0168-3659 (Print)
IS  - 0168-3659 (Linking)
VI  - 142
IP  - 3
DP  - 2010 Mar 19
TI  - Regulating MCP-1 diffusion in affinity hydrogels for enhancing immuno-isolation.
PG  - 384-91
LID - 10.1016/j.jconrel.2009.11.022 [doi]
AB  - Delivering cells using semi-permeable hydrogels is becoming an increasingly 
      important direction in cell based therapies and regenerative medicine 
      applications. Synthetic hydrogels have been functionalized with bioactive motifs 
      to render otherwise inert polymer networks responsive. However, little effort has 
      been focused on creating immuno-isolating materials capable of retarding the 
      transport of small antigenic molecules secreted from the cells delivered with the 
      synthetic carriers. Toward the goal of developing a complete immuno-isolation 
      polymeric barrier, affinity peptide-functionalized PEG hydrogels were developed 
      with the ability to sequester monocyte chemotactic protein 1 (MCP-1), a chemokine 
      known to induce the chemotaxis of monocytes, dendritic cells, and memory T-cells. 
      Affinity peptides capable of sequestering MCP-1 were identified from CCR2 (a G 
      protein-coupled receptor for MCP-1) and incorporated within PEG hydrogels via a 
      thiol-acrylate photopolymerization. The release of encapsulated recombinant MCP-1 
      from PEG hydrogels is readily tuned by: (1) incorporating affinity peptides 
      within the network; and/or (2) altering the spacer distance between the affinity 
      peptide and the crosslinking site. Furthermore, when pancreatic beta-cells were 
      encapsulated within these novel peptide-functionalized hydrogels, the release of 
      cell-secreted MCP-1 was significantly reduced, demonstrating the potential of 
      this new gel formulation to reduce the host innate immune response to 
      transplanted cells by decreasing the recruitment and activation of host monocytes 
      and other immune cells.
CI  - (c) 2009 Elsevier B.V. All rights reserved.
FAU - Lin, Chien-Chi
AU  - Lin CC
AD  - Department of Chemical and Biological Engineering, University of Colorado, 424 
      UCB, Boulder, CO 80309, USA.
FAU - Boyer, Patrick D
AU  - Boyer PD
FAU - Aimetti, Alex A
AU  - Aimetti AA
FAU - Anseth, Kristi S
AU  - Anseth KS
LA  - eng
GR  - R01 DK076084/DK/NIDDK NIH HHS/United States
GR  - R01 DK076084-03/DK/NIDDK NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - R01DK076084/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20091129
PL  - Netherlands
TA  - J Control Release
JT  - Journal of controlled release : official journal of the Controlled Release 
      Society
JID - 8607908
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Hydrogels)
RN  - 0 (Oligopeptides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (poly(ethylene glycol)diacrylate)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Survival
MH  - Chemokine CCL2/chemistry/*immunology/metabolism
MH  - Chemotaxis/drug effects/immunology
MH  - Cross-Linking Reagents/chemistry
MH  - Diabetes Mellitus, Type 1/immunology/therapy
MH  - Hydrogels/*chemistry/pharmacology
MH  - Immunity, Innate/drug effects
MH  - Insulin-Secreting Cells/cytology/*immunology/metabolism/transplantation
MH  - Macrophages/drug effects/immunology
MH  - Mice
MH  - Oligopeptides/*chemistry/pharmacology
MH  - Polyethylene Glycols/*chemistry/pharmacology
MH  - Protein Binding
MH  - Recombinant Proteins/chemistry/immunology/metabolism
PMC - PMC2862573
MID - NIHMS189560
EDAT- 2009/12/03 06:00
MHDA- 2010/06/16 06:00
PMCR- 2010/09/19
CRDT- 2009/12/03 06:00
PHST- 2009/09/23 00:00 [received]
PHST- 2009/11/06 00:00 [revised]
PHST- 2009/11/22 00:00 [accepted]
PHST- 2009/12/03 06:00 [entrez]
PHST- 2009/12/03 06:00 [pubmed]
PHST- 2010/06/16 06:00 [medline]
PHST- 2010/09/19 00:00 [pmc-release]
AID - S0168-3659(09)00800-1 [pii]
AID - 10.1016/j.jconrel.2009.11.022 [doi]
PST - ppublish
SO  - J Control Release. 2010 Mar 19;142(3):384-91. doi: 10.1016/j.jconrel.2009.11.022. 
      Epub 2009 Nov 29.