PMID- 19951957
OWN - NLM
STAT- MEDLINE
DCOM- 20100520
LR  - 20171116
IS  - 1460-2377 (Electronic)
IS  - 0953-8178 (Linking)
VI  - 22
IP  - 2
DP  - 2010 Feb
TI  - CD16/32-specific biotinylated 2.4G2 single-chain Fv complexed with avidin-FITC 
      enhances FITC-specific humoral immune response in vivo in a CD16-dependent 
      manner.
PG  - 71-80
LID - 10.1093/intimm/dxp115 [doi]
AB  - Fcgamma receptors (FcgammaRs) play an essential role in the regulation of immune 
      response due to their ability to bind immune complexes. Activating FcgammaRs may 
      facilitate antigen presentation and dendritic-cell maturation, while in the late 
      phase of the immune response, the inhibitory FcgammaRIIb may down-regulate B-cell 
      activation upon cross-linking with activating receptors. In this study, we 
      investigated the in vivo role of FcgammaRs on the modulation of humoral immune 
      response. In order to get well-defined immune complexes that can bind to both the 
      activating and the inhibitory FcgammaRs, we designed a mono-biotinylated 
      single-chain fragment variable construct from the rat anti-mouse CD16/32 clone 
      2.4G2, linked to avidin-FITC, and tested its effect on the FITC-hapten-specific 
      T-independent type 2 (TI-2) and T-dependent (TD) immune response. When injected 
      intravenously in mice, the complex bound to a small portion of B220+, CD11b(high) 
      and CD11c(high) cells and was localized in the spleen on marginal zone 
      macrophages 15 min after treatment. When applied as a booster following primary 
      immunization with TI-2 (FITC-dextran) or TD (FITC-keyhole limpet haemocyanin) 
      antigens, the complex elevated the number of hapten-specific IgM/IgG-producing B 
      cells. This effect was diminished in CD16KO mice, suggesting that the 
      activating-type FcgammaRIII might be a key mediator of this mechanism.
FAU - Angyal, Adrienn
AU  - Angyal A
AD  - Department of Immunology, Eotvos Lorand University, 1117 Budapest, Hungary.
FAU - Szekeres, Zsuzsanna
AU  - Szekeres Z
FAU - Balogh, Peter
AU  - Balogh P
FAU - Neer, Zsuzsa
AU  - Neer Z
FAU - Szarka, Eszter
AU  - Szarka E
FAU - Virag, Viktor
AU  - Virag V
FAU - Medgyesi, David
AU  - Medgyesi D
FAU - Prechl, Jozsef
AU  - Prechl J
FAU - Sarmay, Gabriella
AU  - Sarmay G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091130
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Cytokines)
RN  - 0 (Dextrans)
RN  - 0 (Fcgr2b protein, mouse)
RN  - 0 (Fcgr3 protein, mouse)
RN  - 0 (Receptors, IgG)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Single-Chain Antibodies)
RN  - 0 (fluorescein isothiocyanate dextran)
RN  - 0 (fluorescein isothiocyante avidin)
RN  - 1405-69-2 (Avidin)
RN  - 9013-72-3 (Hemocyanins)
RN  - FV4Y0JO2CX (keyhole-limpet hemocyanin)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Animals
MH  - Antibody-Producing Cells/drug effects/*immunology
MH  - Avidin/*immunology
MH  - B-Lymphocytes/immunology
MH  - Biotinylation
MH  - Cytokines/metabolism
MH  - Dextrans/administration & dosage/immunology
MH  - Fluorescein-5-isothiocyanate/administration & dosage/*analogs & derivatives
MH  - Hemocyanins/administration & dosage/immunology
MH  - Hybridomas
MH  - *Immunity, Humoral/drug effects
MH  - Injections, Intravenous
MH  - Macrophages/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Protein Binding
MH  - Rats
MH  - Receptors, IgG/deficiency/genetics/*immunology
MH  - Recombinant Fusion Proteins/immunology
MH  - Single-Chain Antibodies/administration & dosage/genetics/*immunology
MH  - Spleen/immunology
MH  - Time Factors
EDAT- 2009/12/03 06:00
MHDA- 2010/05/21 06:00
CRDT- 2009/12/03 06:00
PHST- 2009/12/03 06:00 [entrez]
PHST- 2009/12/03 06:00 [pubmed]
PHST- 2010/05/21 06:00 [medline]
AID - dxp115 [pii]
AID - 10.1093/intimm/dxp115 [doi]
PST - ppublish
SO  - Int Immunol. 2010 Feb;22(2):71-80. doi: 10.1093/intimm/dxp115. Epub 2009 Nov 30.