PMID- 19953904
OWN - NLM
STAT- MEDLINE
DCOM- 20100205
LR  - 20131121
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 89
IP  - 21
DP  - 2009 Jun 2
TI  - [The effects of testosterone on the expression of inflammatory factors in 3T3-L1 
      adipocytes and its mechanism].
PG  - 1493-7
AB  - OBJECTIVE: To explore the effects of androgen upon the production of inflammatory 
      Cultured factors in 3T3-L1 adipocytes and to investigate the mechanism at the 
      molecular level. METHODS: pre-adipocytes from 3T3-L1 cell line were induced to 
      differentiate into adipocytes. Mature adipocytes were treated with testosterone 
      at a concentration of 10(-9) to 10(-15) mol/L for either short (0 to 30 min) or 
      long (12, 24 and 48 h) treatment course. Secretion of inflammatory factors, i.e., 
      interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1), in the 
      culture medium were determined by enzyme-linked immunosorbent assay (ELISA). The 
      mRNA expression of IL-6 and MCP-1 were determined by reverse transcriptase PCR. 
      Phosphorylation of NF-KB was analyzed by Western blot with beta-actin as the 
      reference gene. In another experiment, adipocytes were manipulated according to 
      the same protocol except being pretreated with PDTC (inhibitor of NF-KB) for 2 h 
      prior to the addition of testosterone. The results of two experiments were 
      compared. RESULTS: (1) The secretion of IL-6 and MCP-1 in the culture medium were 
      higher in the testosterone-treated groups than in the control groups (P < 0.05). 
      The highest concentration of IL-6 and MCP-1 were observed in the group treated 
      with 10(-5) M testosterone for 24 h. The mRNA expression of IL-6 and MCP-1 were 
      higher in the groups treated with testosterone for 12 h, especially with 
      testosterone of 10(-5) mol/L; (2) With a short treatment course of testosterone, 
      more NF-kappaB were phosphorylated than in control, especially with testosterone 
      of 10(-5) mol/L. More NF-kappaB was phosphorylated following the 12 h 
      testosterone treatment (10(-9), 10(-7) and 10(-5) mol/L), especially with a 
      testosterone concentration of 10(-9) and 10(-5) mol/L. (3) When pretreated by 
      NF-kappaB inhibitor and followed by 10(-5) mol/L testosterone for 24 h, the 
      secretion of IL-6 and MCP-1 in the culture medium decreased significantly (P < 
      0.01). Likewise, when pretreated by NF-kappaB inhibitor and followed by 10(-5) M 
      testosterone for 12 h, the mRNA expression of IL-6 and MCP-1 decreased 
      significantly. CONCLUSION: Within the certain scope, testosterone could increase 
      the expression of inflammatory factors in adipocytes through the activation of 
      NF-kappaB.
FAU - Su, Chun-lin
AU  - Su CL
AD  - Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
FAU - Huang, Hai-yan
AU  - Huang HY
FAU - Shen, Zong-qi
AU  - Shen ZQ
FAU - Lin, Jin-fang
AU  - Lin JF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*drug effects/*metabolism
MH  - Animals
MH  - Cell Differentiation/drug effects
MH  - Chemokine CCL2/*metabolism
MH  - Interleukin-6/*metabolism
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - Testosterone/*pharmacology
EDAT- 2009/12/04 06:00
MHDA- 2010/02/06 06:00
CRDT- 2009/12/04 06:00
PHST- 2009/12/04 06:00 [entrez]
PHST- 2009/12/04 06:00 [pubmed]
PHST- 2010/02/06 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2009 Jun 2;89(21):1493-7.