PMID- 19953940
OWN - NLM
STAT- MEDLINE
DCOM- 20110929
LR  - 20091203
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 44
IP  - 6
DP  - 2009 Jun
TI  - [Application of sperm fluorescence in situ hybridization in preimplantation 
      genetic diagnosis].
PG  - 418-21
AB  - OBJECTIVE: To investigate the role of sperm fluorescence in situ hybridization 
      (FISH) in preimplantation genetic diagnosis (PGD) for male chromosomal disorders. 
      METHODS: From Jul. 2006 to Aug. 2008, FISH was performed in sperm and embryo of 9 
      infertile couples due to male chromosomal abnormality including 7 couples with 
      Robertsonian translocation, one couple with reciprocal translocation and one 
      couple with Klinefelter's syndrome. Correlation analysis was performed between 
      sperm and embryo FISH results. RESULTS: (1) FISH analysis of 8568 sperms showed 
      24 sperms had no fluorescence signals. The rate of normal/balanced sperm of 
      carriers were 85.71% (6045/7053)in seven Robertsonian translocation, 30.42% 
      (306/1006) in one reciprocal translocation and 68.76% (350/509) in Klinefelter's 
      syndrome. (2) A total of 158 embryos were biopsied, of which 135 embryos were 
      successfully fixed for FISH. A hundred and one embryos exhibit informative signal 
      including 36 normal/balanced embryos and 75 abnormal embryos. Twenty-one embryos 
      were transferred and one couple obtained successful term pregnancy. The rate of 
      normal/balanced embryo were 29.0% (31/107) in 7 carriers of Robertsonian 
      translocation, 6.3% (1/16) in one reciprocal translocation and 33.3% (4/12) in 
      Klinefelter's syndrome. (3) A positive correlated relationship was found between 
      the percentage of normal embryo and the percentage of normal sperm (r = 0.75, P = 
      0.02). CONCLUSION: It is advisable to recommend the sperm FISH analysis for being 
      routinely incorporated into the genetic screening offered prior to 
      preimplantation genetic diagnosis.
FAU - Li, Gang
AU  - Li G
AD  - Reproductive Medical Center, First Affiliated Hospital, Zhengzhou University, 
      Zhengzhou, China.
FAU - Sun, Ying-Pu
AU  - Sun YP
FAU - Jin, Hai-Xia
AU  - Jin HX
FAU - Xin, Zhi-Min
AU  - Xin ZM
FAU - Dai, Shan-Jun
AU  - Dai SJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
SB  - IM
MH  - Chromosome Disorders/*diagnosis
MH  - Embryo Transfer
MH  - Female
MH  - Fertilization in Vitro
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infertility, Male/*diagnosis/etiology
MH  - Karyotyping
MH  - Male
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - *Preimplantation Diagnosis
MH  - Sperm Count
MH  - *Spermatozoa/physiology
MH  - Translocation, Genetic
EDAT- 2009/12/04 06:00
MHDA- 2011/10/01 06:00
CRDT- 2009/12/04 06:00
PHST- 2009/12/04 06:00 [entrez]
PHST- 2009/12/04 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2009 Jun;44(6):418-21.