PMID- 19954619
OWN - NLM
STAT- MEDLINE
DCOM- 20110609
LR  - 20091203
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 30
IP  - 9
DP  - 2009 Sep
TI  - [CpG-oligodeoxynucleotide stimulation improves the success for karyotypic 
      analysis of chronic lymphocytic leukemia cells].
PG  - 601-4
AB  - OBJECTIVE: To explore the effect of CpG-oligodeoxynucleotides (ODN) in chromosome 
      study of chronic lymphocytic leukemia (CLL). METHODS: Blood or bone marrow cells 
      of 70 CLL patients were cultured for 72 h with PHA, CpG-ODN and CpG-ODN combined 
      with IL-2, respectively. Routine karyotype analysis with R banding technique and 
      interphase fluorescence in situ hybridization (FISH) were performed. RESULTS: The 
      metaphase number>or=20 was considered as successful stimulation, which in PHA, 
      CpG-ODN and CpG-ODN combined IL-2 groups were 90.0%, 68.6% and 68.6%, 
      respectively, and the detection rates of chromosome aberrations were 3.2%, 43.6% 
      and 43.6%, respectively. The aberrations rates detected by interphase FISH with a 
      panel of probes was 64.3%. CONCLUSION: CpG-ODN DSP30 can effectively raise the 
      detection rate of chromosome aberrations in CLL patients.
FAU - Liu, Qiong
AU  - Liu Q
AD  - Department of Hematology, the First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, China.
FAU - Xu, Wei
AU  - Xu W
FAU - Qiu, Hai-rong
AU  - Qiu HR
FAU - Wang, Rong
AU  - Wang R
FAU - Yu, Hui
AU  - Yu H
FAU - Fan, Lei
AU  - Fan L
FAU - Miao, Kou-rong
AU  - Miao KR
FAU - Li, Jian-yong
AU  - Li JY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (CpG-ODN DSP30)
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - Chromosome Banding
MH  - Female
MH  - Humans
MH  - Karyotyping
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Oligonucleotides/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 2009/12/04 06:00
MHDA- 2011/06/10 06:00
CRDT- 2009/12/04 06:00
PHST- 2009/12/04 06:00 [entrez]
PHST- 2009/12/04 06:00 [pubmed]
PHST- 2011/06/10 06:00 [medline]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2009 Sep;30(9):601-4.