PMID- 19955267
OWN - NLM
STAT- MEDLINE
DCOM- 20100329
LR  - 20211020
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 48
IP  - 2
DP  - 2010 Feb
TI  - Regional profiling for determination of genotype diversity of mastitis-specific 
      Staphylococcus aureus lineage in Canada by use of clumping factor A, pulsed-field 
      gel electrophoresis, and spa typing.
PG  - 375-86
LID - 10.1128/JCM.01768-09 [doi]
AB  - One of the major concerns in global public health and the dairy industry is the 
      emergence of host-specific virulent Staphylococcus aureus strains. The high 
      degree of stability of the species genome renders detection of genetic 
      microvariations difficult. Thus, approaches for the rapid tracking of specialized 
      lineages are urgently needed. We used clumping factor A (clfA) to profile 87 
      bovine mastitis isolates from four regions in Canada and compared the results to 
      those obtained by pulsed-field gel electrophoresis (PFGE) and spa typing. 
      Twenty-five pulsotypes were obtained by PFGE with an index of discrimination of 
      0.91. These were assigned to six PFGE lineage groups A to F and seven spa types, 
      including two novel ones. Group A had 48.3% of the isolates and group D had 43.7% 
      of the isolates, while only 8% of the isolates were variable. The results of 
      antimicrobial susceptibility testing indicated that all isolates were sensitive 
      to methicillin and the non-beta-lactam antibiotics, while three isolates were 
      resistant to penicillin and one isolate was resistant to tetracycline. All 
      isolates had the clfA gene and belonged to 20 clfA repeat types with an index of 
      discrimination of 0.90. The dominant clfA types, types X, Q, C, and Z, formed 82% 
      and 43% of PFGE groups A and D, respectively, and had copy numbers that varied 
      only within a narrow range of between 46 and 52 copies, implying clonal 
      selection. The rest were variable and region specific. Furthermore, the dominant 
      groups contained subpopulations in different regions across Canada. Sequence 
      information confirmed the relatedness obtained by the use of clfA repeat copy 
      numbers and other methods and further revealed the occurrence of full-repeat 
      deletions and conserved host-specific codon-triplet position biases at 18-bp 
      units. Thus, concordant with the results of PFGE and spa typing, clfA typing 
      proved useful for revealing the clonal nature of the mastitis isolate lineage and 
      for the rapid profiling of subpopulations with comparable discriminatory powers.
FAU - Said, Kamaleldin B
AU  - Said KB
AD  - McGill University, Montreal, Quebec, Canada.
FAU - Ismail, Johanne
AU  - Ismail J
FAU - Campbell, Jennifer
AU  - Campbell J
FAU - Mulvey, Michael R
AU  - Mulvey MR
FAU - Bourgault, Anne-Marie
AU  - Bourgault AM
FAU - Messier, Serge
AU  - Messier S
FAU - Zhao, Xin
AU  - Zhao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091202
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Coagulase)
RN  - 0 (Staphylococcal Protein A)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - *Bacterial Typing Techniques
MH  - Canada
MH  - Cattle
MH  - Cluster Analysis
MH  - Coagulase/*genetics
MH  - *DNA Fingerprinting
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Female
MH  - Genetic Variation
MH  - Genotype
MH  - Mastitis, Bovine/*microbiology
MH  - Microbial Sensitivity Tests
MH  - Staphylococcal Infections/microbiology/*veterinary
MH  - Staphylococcal Protein A/*genetics
MH  - Staphylococcus aureus/*classification/drug effects/genetics/isolation & 
      purification
PMC - PMC2815643
EDAT- 2009/12/04 06:00
MHDA- 2010/03/30 06:00
PMCR- 2010/08/01
CRDT- 2009/12/04 06:00
PHST- 2009/12/04 06:00 [entrez]
PHST- 2009/12/04 06:00 [pubmed]
PHST- 2010/03/30 06:00 [medline]
PHST- 2010/08/01 00:00 [pmc-release]
AID - JCM.01768-09 [pii]
AID - 1768-09 [pii]
AID - 10.1128/JCM.01768-09 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2010 Feb;48(2):375-86. doi: 10.1128/JCM.01768-09. Epub 2009 Dec 
      2.