PMID- 19955845
OWN - NLM
STAT- MEDLINE
DCOM- 20100318
LR  - 20131121
IS  - 1423-0313 (Electronic)
IS  - 0031-7012 (Linking)
VI  - 85
IP  - 1
DP  - 2010
TI  - Curcumin attenuates glucose-induced monocyte chemoattractant protein-1 synthesis 
      in aortic endothelial cells by modulating the nuclear factor-kappaB pathway.
PG  - 18-26
LID - 10.1159/000262325 [doi]
AB  - BACKGROUND/AIMS: High glucose (HG) induces monocyte chemoattractant protein-1 
      (MCP-1) synthesis in endothelial cells through nuclear factor kappaB (NFkappaB). 
      We investigated whether curcumin, losartan and sodium salicylate (NaSal) 
      attenuate HG-induced MCP-1 synthesis in rat aortic endothelial cells (RAECs) and 
      explored the mechanism of action. METHODS: RAECs were stimulated with HG (25 
      mmol/l) for 24 h in the presence or absence of curcumin, losartan, NaSal or 
      NFkappaB inhibitor, Bay 11-0782. The MCP-1 protein and mRNA levels were 
      determined by enzyme-linked immunosorbent assay and real-time reverse 
      transcriptase-polymerase chain reaction, respectively. Nuclear translocation of 
      NFkappaB subunit p65 and NFkappaB DNA-binding activity was studied using confocal 
      microscopy and electrophoretic mobility shift assay, respectively. RESULTS: A 
      significant increase in the synthesis of MCP-1 protein and mRNA (2-fold) was 
      observed in HG-primed RAECs compared to control glucose (5.5 mmol/l). Curcumin 
      (30 micromol/l) significantly decreased HG-induced MCP-1 protein (74%) and mRNA 
      (53%) synthesis. There was no inhibition of HG-induced MCP-1 protein secretion by 
      losartan and NaSal. In HG-stimulated RAECs, curcumin attenuated the nuclear 
      translocation of p65 and decreased the NFkappaB DNA-binding activity. CONCLUSION: 
      Curcumin blocks HG-induced MCP-1 synthesis in RAECs partly via the NFkappaB 
      pathway.
CI  - Copyright 2009 S. Karger AG, Basel.
FAU - Panicker, Sumith Retnamma
AU  - Panicker SR
AD  - Division of Cellular and Molecular Cardiology, Sree Chitra Tirunal Institute for 
      Medical Sciences and Technology, Thiruvananthapuram, India.
FAU - Kartha, Chandrasekharan Cheranellore
AU  - Kartha CC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091201
PL  - Switzerland
TA  - Pharmacology
JT  - Pharmacology
JID - 0152016
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Chemokine CCL2)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - IT942ZTH98 (Curcumin)
RN  - IY9XDZ35W2 (Glucose)
RN  - JMS50MPO89 (Losartan)
RN  - WIQ1H85SYP (Sodium Salicylate)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/pharmacology
MH  - Aorta/cytology/drug effects/metabolism
MH  - Chemokine CCL2/biosynthesis/*drug effects
MH  - Curcumin/*pharmacology
MH  - Endothelium, Vascular/drug effects/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glucose/administration & dosage/*pharmacology
MH  - Losartan/pharmacology
MH  - Male
MH  - NF-kappa B/*metabolism
MH  - RNA, Messenger/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sodium Salicylate/pharmacology
EDAT- 2009/12/04 06:00
MHDA- 2010/03/20 06:00
CRDT- 2009/12/04 06:00
PHST- 2009/07/14 00:00 [received]
PHST- 2009/09/03 00:00 [accepted]
PHST- 2009/12/04 06:00 [entrez]
PHST- 2009/12/04 06:00 [pubmed]
PHST- 2010/03/20 06:00 [medline]
AID - 000262325 [pii]
AID - 10.1159/000262325 [doi]
PST - ppublish
SO  - Pharmacology. 2010;85(1):18-26. doi: 10.1159/000262325. Epub 2009 Dec 1.