PMID- 1995706
OWN - NLM
STAT- MEDLINE
DCOM- 19910322
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 136
IP  - 1
DP  - 1991 Jan 24
TI  - The use of recombinant antigens in ELISA procedures for the quantification of 
      intrathecally produced HIV-1-specific antibodies.
PG  - 133-7
AB  - An ELISA procedure is described for the quantification of intrathecally 
      synthesized immunoglobulin G antibodies to human immunodeficiency virus (HIV) 
      antigens. Recombinant p17, p24, endonuclease (END), reverse transcriptase (RT), a 
      peptide from the transmembrane region of gp41 (ENV80) and a fusion protein 
      containing HIV-1 and HIV-2 epitopes were compared with a commercially available 
      ELISA. Using a reference serum, antibodies in serum and cerebrospinal fluid (CSF) 
      to all of the antigens could be measured quantitatively in a reliable and 
      reproducible fashion. Despite the fact that the titer varied up to 10(5)-fold 
      between CSF and serum, interassay variability ranged from 3.87% for p17 to 8.41% 
      for RT and intra-assay variability varied from 3.9% +/- 1.2% for p17 to 14.3% +/- 
      3.9% for the commercial ELISA. Antibody specificity indices (ASI) obtained by 
      relating CSF/serum titers with reference to the corresponding IgG concentrations 
      can be used to detect intrathecal synthesis of virus specific antibodies.
FAU - Weber, T
AU  - Weber T
AD  - Department of Neurology, University of Gottingen, F.R.G.
FAU - Freter, A
AU  - Freter A
FAU - Luer, W
AU  - Luer W
FAU - Haas, J
AU  - Haas J
FAU - Stark, E
AU  - Stark E
FAU - Poser, S
AU  - Poser S
FAU - Felgenhauer, K
AU  - Felgenhauer K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (HIV Antibodies)
RN  - 0 (HIV Antigens)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Enzyme-Linked Immunosorbent Assay
MH  - HIV Antibodies/*analysis
MH  - HIV Antigens/*immunology
MH  - HIV-1/*immunology
MH  - Humans
MH  - Immunoglobulin G/cerebrospinal fluid
MH  - Recombinant Proteins/immunology
MH  - Spinal Cord/*immunology
EDAT- 1991/01/24 00:00
MHDA- 1991/01/24 00:01
CRDT- 1991/01/24 00:00
PHST- 1991/01/24 00:00 [pubmed]
PHST- 1991/01/24 00:01 [medline]
PHST- 1991/01/24 00:00 [entrez]
AID - 0022-1759(91)90259-I [pii]
AID - 10.1016/0022-1759(91)90259-i [doi]
PST - ppublish
SO  - J Immunol Methods. 1991 Jan 24;136(1):133-7. doi: 10.1016/0022-1759(91)90259-i.