PMID- 19957534
OWN - NLM
STAT- MEDLINE
DCOM- 20100202
LR  - 20151006
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 89
IP  - 24
DP  - 2009 Jun 23
TI  - [Experimental identification of drugs with function of targeted up-regulating ID4 
      expression: bioinformatics-based prediction and preliminary validation].
PG  - 1714-6
AB  - OBJECTIVE: To screen new candidate molecular-targeted anti-leukemia compounds 
      with potential functions of targeted up-regulating ID4 gene expression. METHODS: 
      Promoter region of ID4 gene including the upstream - 3000 bp sequence of 
      transcriptional start site and message RNA sequence were fished out. Online 
      promoter analysis tools of TESS and Genomax were used to search possible sequence 
      of transcriptional start site and message RNA sequence were fished out. Online 
      promoter analysis tools of TESS and Genomax were used to search possible 
      cis-acting structure from human transcription factor database. The activity of 
      related drugs with potential effects upon ID4 gene expression was analyzed using 
      SAGE database. GEO database was applied to search the gene expression profiling 
      regulated by ID4 gene. Finally, similar analysis between gene expression 
      profiling by ID4 and genome-wide profiling regulated by 163 known drugs or active 
      compounds was manipulated to screen the drugs and candidate compounds with 
      similar gene expression profiling with ID4 gene. MOLT4 cell line was treated with 
      the above candidate active compounds to investigate the ID4 gene expression by 
      RT-PCR assay. RESULTS: ID4 gene had a type II promoter with a typical TATA box in 
      upstream -45 bp of transcription start site. The 1300 bp-length promoter of ID4 
      gene contained a few cis-acting structures classified into two function types, i. 
      e. positive regulatory type, including transcription factors Spl and c-Myb, cAMP, 
      glucocorticoid receptor (GR) and estrogen receptor (ER), and negative regulatory 
      type, including Wilms tumor-1 (WT1) and early growth response-2 (EGR2). The 
      similarity of gene expression profiling was identified between cAMP and ID4 gene. 
      ID4 gene expression was induced in MOLT4 cell line after treatment with calcium 
      dibutyryladenosine cyclophosphate at the concentration of 0.1 mmol/L. CONCLUSION: 
      The comprehensive bioinformatic analysis, based upon the combination of 
      regulatory sequence prediction of promoter, similarity analysis of gene 
      expression profiling and literature review, can be considered as a practical tool 
      in screening the candidate drugs with the activity of targeted regulating 
      functional genes. Calcium dibutyryladenosine cyclophosphate can induce ID4 gene 
      expression in leukemic cells.
FAU - Yang, Bo
AU  - Yang B
AD  - Department of Geriatric Hematology, Chinese PLA General Hospital, Beijing 100853, 
      China.
FAU - Lu, Xue-Chun
AU  - Lu XC
FAU - Liu, Li-Hong
AU  - Liu LH
FAU - Zhu, Hong-Li
AU  - Zhu HL
FAU - Chi, Xiao-Hua
AU  - Chi XH
FAU - Yao, Shan-Qian
AU  - Yao SQ
FAU - Lou, Fang-Ding
AU  - Lou FD
FAU - Yu, Li
AU  - Yu L
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (ID4 protein, human)
RN  - 0 (Inhibitor of Differentiation Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cell Line, Tumor
MH  - *Computational Biology
MH  - Humans
MH  - Inhibitor of Differentiation Proteins/*genetics
MH  - RNA, Messenger/genetics
MH  - Regulatory Sequences, Nucleic Acid
MH  - Sequence Analysis, DNA
MH  - Up-Regulation
EDAT- 2009/12/05 06:00
MHDA- 2010/02/03 06:00
CRDT- 2009/12/05 06:00
PHST- 2009/12/05 06:00 [entrez]
PHST- 2009/12/05 06:00 [pubmed]
PHST- 2010/02/03 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2009 Jun 23;89(24):1714-6.