PMID- 19957853
OWN - NLM
STAT- MEDLINE
DCOM- 20100504
LR  - 20091204
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 23
IP  - 10
DP  - 2009 Oct
TI  - [Property studies on three-dimensional porous blended silk scaffolds].
PG  - 1264-70
AB  - OBJECTIVE: To explore the differences of three-dimensional porous blended silk 
      scaffolds with different sericin ratios in terms of molecular structure, 
      mechanical properties, and biological characteristics. METHODS: Fibroin/sericin 
      blended aqueous solution [concentration 8% (W/V)] with various sericin ratios 0%, 
      2%, 4%, 6%, 8%, 10%, 12% and NaCl as a porogen with different particle sizes 
      (125-200, 200-300, 300-450, 450-600, 600-900, 900-1100 microm) were used to 
      fabricate the three-dimensional porous blended silk scaffolds. Gross observation 
      of the formation of three-dimensional porous blended silk scaffolds of different 
      sericin ratios and pore sizes was performed. Scanning electron microscope (SEM) 
      was used to detect the distribution and diameter of the pore sizes. Its porosity 
      was calculated by liquids replacement method. X-ray diffractometer (XRD) and 
      fourier transform infrared (FTIR) were used to detect its internal molecular 
      structure. Its mechanical properties, enzyme degration rate in vitro and 
      experiment on SD rats in vivo, and histology observation after coculturing 
      homogeneous scaffold (sericin ratio 0-12%, NaCl particle size 600-900 microm) 
      with adipose tissue-derived mesenchymal stem cells (ADSCs) were detected. 
      RESULTS: Gross observation showed that the higher of the ratio of sericin 
      protein, the greater of the porogen sizes scope which used to form homogeneous 
      silk scaffolds. The result of SEM showed that the pores of the three-dimensional 
      porous blended silk scaffolds had uniform distribution and was connected with 
      each other. Its pore sizes was in the scope of the porogen sizes, and its 
      porosity all above 90%. The angel corresponding to the characteristic peak of the 
      sericin/fibroin blended scaffolds were 20.6 degrees and 24.6 degrees (XRD), and 
      the wavelength corresponding to the characteristic peak of the sericin/fibroin 
      blended scaffolds were 3 296, 2 933 and 1629 cm(-1) (FTIR) which was the same as 
      the angel and wavelength corresponding to the characteristic peak of the natural 
      silk. The mechanical properties of the sericin/fibroin blended scaffolds was 
      improved with the increase of sericin ratios, and the compressional resilience 
      reached 100% when the ratio > or = 6%. The different ratios of sericin and the 
      different particle size of porogen had no significant effect on the enzyme 
      degradation rate in vitro. The histological observation 14 days after 
      ADSCs-scaffold co-culture indicated that the scaffolds had slow degradation rate, 
      and slight inflammatory response in vivo. ADSCs were well attached to the 
      sericin/fibroin blended scaffolds of different sericin ratios, with varied 
      morphology, rich cytoplasm, and nuclear enrichment, the light staining ECM was 
      observed surrounding the cells. CONCLUSION: The mechanical property of the 
      three-dimensional porous blended silk scaffolds is improved by silk sericins with 
      ratio > or = 6% obviously, which will lay the groudwork for further research and 
      making of strengthen silk scaffolds.
FAU - Rao, Jianwei
AU  - Rao J
AD  - Department of Plastic and Reconstructive Surgery, First Hospital Affiliated to 
      Sun Yat-sen University, Guangzhou Guangdong, 510080, P.R. China.
FAU - Shen, Jun
AU  - Shen J
FAU - Quan, Daping
AU  - Quan D
FAU - Xu, Yangbin
AU  - Xu Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Biocompatible Materials)
RN  - 0 (Sericins)
RN  - 9007-76-5 (Fibroins)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials
MH  - *Cytoskeleton
MH  - Female
MH  - Fibroins/*chemistry
MH  - Materials Testing
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sericins/*chemistry
MH  - Tissue Engineering
MH  - Tissue Scaffolds
EDAT- 2009/12/05 06:00
MHDA- 2010/05/05 06:00
CRDT- 2009/12/05 06:00
PHST- 2009/12/05 06:00 [entrez]
PHST- 2009/12/05 06:00 [pubmed]
PHST- 2010/05/05 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009 Oct;23(10):1264-70.