PMID- 19958600
OWN - NLM
STAT- MEDLINE
DCOM- 20100609
LR  - 20220408
IS  - 1945-8932 (Electronic)
IS  - 1945-8932 (Linking)
VI  - 23
IP  - 6
DP  - 2009 Nov-Dec
TI  - Characterization of bacterial and fungal biofilms in chronic rhinosinusitis.
PG  - 556-61
LID - 10.2500/ajra.2009.23.3413 [doi]
AB  - BACKGROUND: Conclusive evidence exists that biofilms are present on the mucosa of 
      chronic rhinosinusitis (CRS) patients. Less is known about the species 
      constituting these biofilms. This study developed a fluorescence in situ 
      hybridization (FISH) protocol for characterization of bacterial and fungal 
      biofilms in CRS. METHODS: Fifty CRS patients and 10 controls were recruited. 
      Bacteria FISH probes for Staphylococcus aureus, Haemophilus influenzae, and 
      Pseudomonas aeruginosa and a universal probe for fungi were applied to sinus 
      mucosal specimens and then analyzed using confocal scanning laser microscopy. 
      RESULTS: Thirty-six of 50 CRS patients had biofilms present in contrast to 0/10 
      controls, suggesting a role for biofilms in the pathogenesis of this disease. S. 
      aureus was the most common biofilm-forming organism. Eleven of 50 CRS patients 
      had characteristic fungal biofilms present. CONCLUSION: This is the largest study 
      of biofilms in CRS. It has validated mucosal tissue cryopreservation for delayed 
      biofilm analysis. Fungal biofilms have been identified and the importance of S. 
      aureus biofilms in the polymicrobial etiology of CRS is highlighted.
FAU - Foreman, Andrew
AU  - Foreman A
AD  - Department of Surgery-Otorhinolaryngology, Head and Neck Surgery, University of 
      Adelaide, Adelaide, Australia.
FAU - Psaltis, Alkis James
AU  - Psaltis AJ
FAU - Tan, Lor Wai
AU  - Tan LW
FAU - Wormald, Peter-John
AU  - Wormald PJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Rhinol Allergy
JT  - American journal of rhinology & allergy
JID - 101490775
SB  - IM
MH  - Adult
MH  - *Biofilms
MH  - Chronic Disease
MH  - Female
MH  - Fungi/*growth & development/pathogenicity
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Mycoses/complications/*diagnosis
MH  - Nasal Mucosa/*microbiology
MH  - Rhinitis/etiology/prevention & control
MH  - Sinusitis/etiology/prevention & control
MH  - Staphylococcal Infections/complications/*diagnosis
MH  - Staphylococcus aureus/*growth & development/pathogenicity
EDAT- 2009/12/05 06:00
MHDA- 2010/06/10 06:00
CRDT- 2009/12/05 06:00
PHST- 2009/12/05 06:00 [entrez]
PHST- 2009/12/05 06:00 [pubmed]
PHST- 2010/06/10 06:00 [medline]
AID - 10.2500/ajra.2009.23.3413 [doi]
PST - ppublish
SO  - Am J Rhinol Allergy. 2009 Nov-Dec;23(6):556-61. doi: 10.2500/ajra.2009.23.3413.