PMID- 19959244
OWN - NLM
STAT- MEDLINE
DCOM- 20100628
LR  - 20190617
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 135
IP  - 1-2
DP  - 2010 May 15
TI  - Protective effect of glutathione S-transferase-fused mutant staphylococcal 
      enterotoxin C against Staphylococcus aureus-induced bovine mastitis.
PG  - 64-70
LID - S0165-2427(09)00387-0 [pii]
LID - 10.1016/j.vetimm.2009.11.001 [doi]
AB  - Recent studies have demonstrated that immunization with nontoxic mutant 
      staphylococcal enterotoxin C (mSEC) provides protection against Staphylococcus 
      aureus infection in mouse models. In the present study, we investigated whether 
      vaccination with a glutathione S-transferase-fused SEC (GST-mSEC) can protect 
      against S. aureus-induced bovine mastitis. Cows were immunized with the GST-mSEC 
      plus alum adjuvant and then challenged with viable S. aureus by an intramammary 
      route. The results showed that immunization with GST-mSEC-induced production of 
      SEC-specific antibodies in sera and the high titers of antibodies could persist 
      for over 12 weeks. Importantly, immunization with GST-mSEC also induced 
      production of SEC-specific antibodies in milk. The somatic cell counts in the 
      milk from S. aureus challenged quarters of vaccinated lactating cows were 
      significantly lower than those of the non-vaccinated control animals. 
      Furthermore, the sera from GST-mSEC-immunized cows significantly inhibited 
      interferon-gamma and tumor necrosis factor-alpha production from mouse spleen 
      cells induced by wild-type SEC. These results suggest that vaccination with 
      GST-mSEC provides protection against S. aureus-induced bovine mastitis and that 
      the protection might be mediated by SEC-neutralizing antibodies.
CI  - Copyright 2009 Elsevier B.V. All rights reserved.
FAU - Cui, Jing-Chun
AU  - Cui JC
AD  - Department of Preventive Veterinary Science, School of Veterinary Medicine, 
      Northeastern Agricultural University, Harbin 150030, PR China; Department of 
      Bio-Engineering, College of Life Science, Dalian Nationalities University, Dalian 
      116620, PR China.
FAU - Zhang, Bao-Jun
AU  - Zhang BJ
AD  - Department of Prevention Veterinary Science, College of Animal Science and 
      Technology, Jilin Agricultural University, Changchun 130118, PR China.
FAU - Lin, Yan-Chun
AU  - Lin YC
AD  - Department of Prevention Veterinary Science, College of Animal Science and 
      Technology, Jilin Agricultural University, Changchun 130118, PR China.
FAU - Wang, Quan-Kai
AU  - Wang QK
AD  - Department of Prevention Veterinary Science, College of Animal Science and 
      Technology, Jilin Agricultural University, Changchun 130118, PR China.
FAU - Qian, Ai-Dong
AU  - Qian AD
AD  - Department of Prevention Veterinary Science, College of Animal Science and 
      Technology, Jilin Agricultural University, Changchun 130118, PR China.
FAU - Nakane, Akio
AU  - Nakane A
AD  - Department of Microbiology and Immunology, Hirosaki University Graduate School of 
      Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan.
FAU - Hu, Dong-Liang
AU  - Hu DL
AD  - Department of Microbiology and Immunology, Hirosaki University Graduate School of 
      Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan. Electronic address: 
      hudl@cc.hirosaki-u.ac.jp.
FAU - Tong, Guang-Zhi
AU  - Tong GZ
AD  - Department of Preventive Veterinary Science, School of Veterinary Medicine, 
      Northeastern Agricultural University, Harbin 150030, PR China; Shanghai 
      Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 
      200241, PR China. Electronic address: gztong@shvri.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091111
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Cytokines)
RN  - 0 (Enterotoxins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Superantigens)
RN  - 0 (Vaccines, Synthetic)
RN  - 39424-54-9 (enterotoxin C, staphylococcal)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/immunology
MH  - Antibody Formation/immunology
MH  - Cattle
MH  - Cytokines/immunology
MH  - Enterotoxins/*immunology/therapeutic use
MH  - Female
MH  - Glutathione Transferase/*immunology/therapeutic use
MH  - Mastitis, Bovine/immunology/*prevention & control
MH  - Milk/immunology
MH  - Recombinant Fusion Proteins/immunology/therapeutic use
MH  - Staphylococcal Infections/immunology/prevention & control/*veterinary
MH  - Staphylococcus aureus/immunology
MH  - Superantigens/immunology
MH  - Vaccines, Synthetic/immunology/*therapeutic use
EDAT- 2009/12/05 06:00
MHDA- 2010/06/29 06:00
CRDT- 2009/12/05 06:00
PHST- 2009/01/30 00:00 [received]
PHST- 2009/10/06 00:00 [revised]
PHST- 2009/11/05 00:00 [accepted]
PHST- 2009/12/05 06:00 [entrez]
PHST- 2009/12/05 06:00 [pubmed]
PHST- 2010/06/29 06:00 [medline]
AID - S0165-2427(09)00387-0 [pii]
AID - 10.1016/j.vetimm.2009.11.001 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2010 May 15;135(1-2):64-70. doi: 
      10.1016/j.vetimm.2009.11.001. Epub 2009 Nov 11.