PMID- 19959493
OWN - NLM
STAT- MEDLINE
DCOM- 20100330
LR  - 20151119
IS  - 1938-2723 (Electronic)
IS  - 1076-0296 (Linking)
VI  - 16
IP  - 1
DP  - 2010 Feb
TI  - Inflammatory biomarker profiling in elderly patients with acute hip fracture 
      treated with heparins.
PG  - 42-50
LID - 10.1177/1076029609351876 [doi]
AB  - Hip fracture is common in the elderly patients with associated high risk of 
      venous thromboembolic complications. Pathogenic activation results in the 
      generation of various surrogate markers in plasma. This study is designed to 
      identify unique biomarkers in elderly patients with hip fracture using protein 
      chip array enzyme-linked immunosorbent assay (ELISA) methods. Plasma from a 
      randomized hip fracture study (PK-532; n = 341) treated with either enoxaparin 
      (40 mg once daily) or unfractionated heparin (UFH; 5000 IU twice daily) were 
      collected prior to and at 1, 3, 5, and 7 days. A total of 52 samples were 
      analyzed using proteomic surface-enhanced laser desorption/ ionization-time of 
      flight (SELDI-TOF) mass spectrometry to identify unique biomarkers in the 
      molecular weight range of 0 to 150 kd. Twenty-nine healthy volunteer's and pooled 
      plasma from total hip replacement/total knee replacement patients with a unique 
      biomarker at 11.9 kd were used as quality controls. In the 29 healthy 
      individuals, the biomarker profile did not reveal the presence of any unique peak 
      in comparison to the reference normal human plasma (NHP). Plasma obtained prior 
      to surgery exhibits unique biomarkers in 4 of 52 (7.6%) of the samples. On day 1 
      postoperatively, 41 of 51 (80.3%) showed a distinct peak at 11.9 kd. On day 3, 43 
      of 49 (87.8%) patients showed the presence of this biomarker most often at its 
      strongest intensity. In all, 22 of 44 (50%) showed this biomarker on day 5 and 4 
      of 23 (17.9%) on day 7. C-reactive protein (CRP), tumor necrosis factor alpha 
      (TNF-alpha), and serum amyloid A were also increased after surgery. Tissue factor 
      pathway inhibitor (TFPI) antigen levels were increased due to the treatment 
      modalities.
FAU - Knesek, Michael John
AU  - Knesek MJ
AD  - Loyola Universtity Stritch School of Medicine Maywood, Illinois 60153, USA.
FAU - Litinas, Evangelos
AU  - Litinas E
FAU - Adiguzel, Cafer
AU  - Adiguzel C
FAU - Hopkinson, William
AU  - Hopkinson W
FAU - Hoppensteadt, Debra
AU  - Hoppensteadt D
FAU - Lassen, Michael
AU  - Lassen M
FAU - Fareed, Jawed
AU  - Fareed J
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20091202
PL  - United States
TA  - Clin Appl Thromb Hemost
JT  - Clinical and applied thrombosis/hemostasis : official journal of the 
      International Academy of Clinical and Applied Thrombosis/Hemostasis
JID - 9508125
RN  - 0 (Acute-Phase Proteins)
RN  - 0 (Anticoagulants)
RN  - 0 (Biomarkers)
RN  - 0 (Enoxaparin)
RN  - 0 (Lipoproteins)
RN  - 0 (Serum Amyloid A Protein)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (lipoprotein-associated coagulation inhibitor)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Acute Disease
MH  - Acute-Phase Proteins/metabolism
MH  - Aged
MH  - Aged, 80 and over
MH  - Anticoagulants/*administration & dosage/adverse effects
MH  - Arthroplasty, Replacement, Hip
MH  - Biomarkers/*blood
MH  - C-Reactive Protein/metabolism
MH  - Enoxaparin/*administration & dosage/adverse effects
MH  - Female
MH  - Hip Fractures/*blood/epidemiology/surgery
MH  - Humans
MH  - Lipoproteins/blood
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
MH  - Protein Array Analysis
MH  - Proteomics
MH  - Risk Factors
MH  - Serum Amyloid A Protein/metabolism
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Thromboembolism/*blood/epidemiology/*prevention & control
MH  - Tumor Necrosis Factor-alpha/blood
EDAT- 2009/12/05 06:00
MHDA- 2010/03/31 06:00
CRDT- 2009/12/05 06:00
PHST- 2009/12/05 06:00 [entrez]
PHST- 2009/12/05 06:00 [pubmed]
PHST- 2010/03/31 06:00 [medline]
AID - 1076029609351876 [pii]
AID - 10.1177/1076029609351876 [doi]
PST - ppublish
SO  - Clin Appl Thromb Hemost. 2010 Feb;16(1):42-50. doi: 10.1177/1076029609351876. 
      Epub 2009 Dec 2.