PMID- 19961888
OWN - NLM
STAT- MEDLINE
DCOM- 20100429
LR  - 20211203
IS  - 1873-5169 (Electronic)
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 31
IP  - 2
DP  - 2010 Feb
TI  - Novel insight in distribution of nesfatin-1 and phospho-mTOR in the arcuate 
      nucleus of the hypothalamus of rats.
PG  - 257-62
LID - 10.1016/j.peptides.2009.11.024 [doi]
AB  - Recently, two proteins have been localized in the arcuate nucleus (ARC) and 
      implicated in the regulation of food intake: the serine-threonine-kinase 
      mammalian target of rapamycin (mTOR) as part of the TOR signaling complex 1 
      (TORC1), and nesfatin-1 derived from the precursor protein nucleobindin2. 
      However, the exact cell types are not well described. Therefore, we performed 
      double-labeling studies for NPY, CART, nesfatin-1 and pmTOR in the ARC. In this 
      study, we showed that nesfatin-1 is not only intracellularly co-localized with 
      cocaine- and amphetamine-regulated transcript (CART) peptide as reported before, 
      but also with phospho-mTOR (pmTOR) and neuropeptide Y (NPY) in ARC neurons. 
      Quantification revealed that 59+/-5% of the pmTOR-immunoreactive (ir) neurons 
      were immunoreactive for nesfatin-1. Moreover, double labeling for nesfatin-1 and 
      NPY exhibited that 19+/-5% of the NPY positive cells were also immunoreactive for 
      nesfatin-1. Furthermore, we could also confirm results from previous studies, 
      showing that the majority of nesfatin-1 neurons are also positive for CART 
      peptide, whereas most of the pmTOR is co-localized with NPY and only to a lesser 
      extent with CART.
CI  - (c) 2009 Elsevier Inc. All rights reserved.
FAU - Inhoff, Tobias
AU  - Inhoff T
AD  - Department of Medicine, Division Hepatology, Gastroenterology, and Endocrinology, 
      Charite, Campus Virchow, Universitatsmedizin Berlin, Germany.
FAU - Stengel, Andreas
AU  - Stengel A
FAU - Peter, Lisa
AU  - Peter L
FAU - Goebel, Miriam
AU  - Goebel M
FAU - Tache, Yvette
AU  - Tache Y
FAU - Bannert, Norbert
AU  - Bannert N
FAU - Wiedenmann, Bertram
AU  - Wiedenmann B
FAU - Klapp, Burghard F
AU  - Klapp BF
FAU - Monnikes, Hubert
AU  - Monnikes H
FAU - Kobelt, Peter
AU  - Kobelt P
LA  - eng
GR  - P30 DK041301/DK/NIDDK NIH HHS/United States
GR  - R01 DK033061/DK/NIDDK NIH HHS/United States
GR  - DK-41301/DK/NIDDK NIH HHS/United States
GR  - NIHDK 33061/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20091202
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Nucb1 protein, rat)
RN  - 0 (Nucleobindins)
RN  - 0 (cocaine- and amphetamine-regulated transcript protein)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Arcuate Nucleus of Hypothalamus/cytology/*metabolism
MH  - Calcium-Binding Proteins
MH  - DNA-Binding Proteins
MH  - Immunohistochemistry
MH  - Intracellular Signaling Peptides and Proteins/*metabolism
MH  - Male
MH  - Microscopy, Confocal
MH  - Microscopy, Fluorescence
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurons/metabolism
MH  - Neuropeptide Y/metabolism
MH  - Nucleobindins
MH  - Phosphorylation
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - TOR Serine-Threonine Kinases
PMC - PMC4043136
MID - NIHMS585532
EDAT- 2009/12/08 06:00
MHDA- 2010/04/30 06:00
PMCR- 2014/06/03
CRDT- 2009/12/08 06:00
PHST- 2009/09/23 00:00 [received]
PHST- 2009/11/24 00:00 [revised]
PHST- 2009/11/24 00:00 [accepted]
PHST- 2009/12/08 06:00 [entrez]
PHST- 2009/12/08 06:00 [pubmed]
PHST- 2010/04/30 06:00 [medline]
PHST- 2014/06/03 00:00 [pmc-release]
AID - S0196-9781(09)00515-4 [pii]
AID - 10.1016/j.peptides.2009.11.024 [doi]
PST - ppublish
SO  - Peptides. 2010 Feb;31(2):257-62. doi: 10.1016/j.peptides.2009.11.024. Epub 2009 
      Dec 2.