PMID- 19962263
OWN - NLM
STAT- MEDLINE
DCOM- 20100330
LR  - 20171116
IS  - 1618-0984 (Electronic)
IS  - 0723-2020 (Linking)
VI  - 33
IP  - 1
DP  - 2010 Jan
TI  - Microbial associations in gut systems of wood- and bark-inhabiting longhorned 
      beetles [Coleoptera: Cerambycidae].
PG  - 25-34
LID - 10.1016/j.syapm.2009.10.002 [doi]
AB  - Using fluorescence in situ hybridization (FISH) techniques and PCR-based rDNA 
      sequencing, gut microflora in the larvae of bark- and wood-inhabiting cerambycid 
      beetles (Rhagium inquisitor, Tetropium castaneum, Plagionotus arcuatus and 
      Leptura rubra [Coleoptera: Cerambycidae]) was investigated. A total of 12 novel 
      ascomycetous yeast strains were isolated from the gut content. Panfungal and 
      strain-specific oligonucleotide probes identified two yeast strains as Candida 
      rhagii and Candida shehatae, which were colonizing specialized organs (mycetomes) 
      adhering to the gut of R. inquisitor and L. rubra larvae, respectively. Fragments 
      containing these organisms were constantly being released from the mycetomes into 
      the gut lumen. Whereas the mycetome symbiont of T. castaneum could not be 
      identified, all larvae of this species harbored an additional bacterial 
      endocytobiont in their gut epithelium. This novel gammaproteobacterium belonged 
      to the Sodalis clade of insect symbionts, which includes the secondary 
      endosymbiont of tsetse flies (Sodalis glossinidius) and the Sitophilus oryzae 
      primary endosymbiont (SOPE). Extracellular gut flora of the investigated 
      cerambycid larvae was comprised of Alpha-, Beta-, and Gammaproteobacteria, 
      Actinobacteria, Firmicutes, Verrucomicrobia and Acidobacteria. However, the 
      individual composition among investigated larvae was highly variable and 
      supposedly depended on individual host nutrition.
CI  - Copyright (c) 2009 Elsevier GmbH. All rights reserved.
FAU - Grunwald, S
AU  - Grunwald S
AD  - Fachgebiet fur Pflanzenphysiologie, Department fur Pflanzenwissenschaften, 
      Technische Universitaet Muenchen, am Hochanger 4, 85354 Freising, Germany. 
      sebastian.gruenwald@zilicon.de
FAU - Pilhofer, M
AU  - Pilhofer M
FAU - Holl, W
AU  - Holl W
LA  - eng
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PT  - Journal Article
DEP - 20091203
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/*classification/*isolation & purification
MH  - *Biodiversity
MH  - Cluster Analysis
MH  - Coleoptera/*microbiology
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Fungi/*classification/*isolation & purification
MH  - Gastrointestinal Tract/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
EDAT- 2009/12/08 06:00
MHDA- 2010/03/31 06:00
CRDT- 2009/12/08 06:00
PHST- 2008/10/15 00:00 [received]
PHST- 2009/12/08 06:00 [entrez]
PHST- 2009/12/08 06:00 [pubmed]
PHST- 2010/03/31 06:00 [medline]
AID - S0723-2020(09)00129-5 [pii]
AID - 10.1016/j.syapm.2009.10.002 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2010 Jan;33(1):25-34. doi: 10.1016/j.syapm.2009.10.002. Epub 
      2009 Dec 3.