PMID- 19962752
OWN - NLM
STAT- MEDLINE
DCOM- 20100408
LR  - 20100129
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 31
IP  - 7
DP  - 2010 Mar
TI  - Fluorescent oligonucleotide probe based on G-quadruplex scaffold for signal-on 
      ultrasensitive protein assay.
PG  - 1918-24
LID - 10.1016/j.biomaterials.2009.11.054 [doi]
AB  - This work reported the G-quadruplex structure of pyrene-labeled G-rich DNA probe 
      and its application in the immunoglobulin E (IgE) detection, providing plausibly 
      an insight into the biological function of human telomere. Based on the 
      intermolecular G-quadruplex, a terminal-single-pyrene-labeled oligonucleotide 
      signaling probe was developed and a novel protein assay strategy was proposed via 
      combining specific DNA cleavage by S1 nuclease with target-recognizing aptamer. 
      This assay platform not only circumvented the optimization of specific sites for 
      reporter attachment and the pyrene monomer fluorescence quenching by flanking 
      nucleotide bases but also presented a signal-on mechanism. Thus, ultrasensitive 
      homogeneous detection of IgE was successfully conducted. A linear dynamic range 
      of 4.72 x 10(-12) to 7.56 x 10(-9)m, a regression coefficient of 0.9941 and a 
      detection limit of 9.45 x 10(-14)m were given. Additionally, a preliminary 
      concept of the single-pyrene-labeled excimer fluorescence probes associated with 
      G-quadruplex for screening biological markers was described. Importantly, the 
      unexpected structural property of G-quadruplex discovered seems to provide 
      valuable information to allow understanding of the structure and function of 
      human telomere and exploring of useful structure-based anticancer drug.
CI  - (c) 2009. Published by Elsevier Ltd. All rights reserved.
FAU - Wu, Zai-Sheng
AU  - Wu ZS
AD  - State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry 
      and Chemical Engineering, Hunan University, Changsha, PR China. 
      wuzaisheng@163.com
FAU - Hu, Peng
AU  - Hu P
FAU - Zhou, Hui
AU  - Zhou H
FAU - Shen, Guoli
AU  - Shen G
FAU - Yu, Ruqin
AU  - Yu R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091204
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Aptamers, Peptide)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Oligonucleotides)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Aptamers, Peptide/metabolism
MH  - Biological Assay/*methods
MH  - Biosensing Techniques
MH  - Fluorescent Dyes/*metabolism
MH  - *G-Quadruplexes
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Immunoglobulin E/*metabolism
MH  - Models, Biological
MH  - Oligonucleotides/*metabolism
MH  - Spectrometry, Fluorescence
MH  - Time Factors
EDAT- 2009/12/08 06:00
MHDA- 2010/04/09 06:00
CRDT- 2009/12/08 06:00
PHST- 2009/10/07 00:00 [received]
PHST- 2009/11/18 00:00 [accepted]
PHST- 2009/12/08 06:00 [entrez]
PHST- 2009/12/08 06:00 [pubmed]
PHST- 2010/04/09 06:00 [medline]
AID - S0142-9612(09)01298-8 [pii]
AID - 10.1016/j.biomaterials.2009.11.054 [doi]
PST - ppublish
SO  - Biomaterials. 2010 Mar;31(7):1918-24. doi: 10.1016/j.biomaterials.2009.11.054. 
      Epub 2009 Dec 4.