PMID- 19963136
OWN - NLM
STAT- MEDLINE
DCOM- 20091217
LR  - 20091207
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 196
IP  - 1
DP  - 2010 Jan 1
TI  - Genetic and immunophenotypic profile of IGH@ rearrangement detected by 
      fluorescence in situ hybridization in 149 cases of B-cell chronic lymphocytic 
      leukemia.
PG  - 56-63
LID - 10.1016/j.cancergencyto.2009.08.021 [doi]
AB  - Recent studies have shown a higher frequency of immunoglobulin heavy (IGH@) locus 
      rearrangement in B-cell chronic lymphocytic leukemia (B-CLL) than previously 
      reported. However, association of the IGH@ rearrangement with specific 
      chromosomal abnormalities and immunophenotypic markers in B-CLL is still under 
      further investigation. In this study, we analyzed 149 bone marrow aspirate or 
      peripheral blood specimens from patients diagnosed with B-CLL, evaluated by four 
      different laboratory studies: morphology examination, three- or four-color flow 
      cytometry analysis, conventional cytogenetics, and fluorescence in situ 
      hybridization (FISH) with a dual-color, break-apart IGH@ probe in addition to a 
      B-CLL FISH probe panel for del(11)(q22) ATM, del(13)(q14.3), del(17)(p13) TP53, 
      and +12. An IGH@ rearrangement was found by FISH in 24 cases (16.0%). Of these 24 
      cases, 16 (67%) contained chromosomal abnormalities, including 
      t(14;19)(q32;q13.2), t(8;14)(q24;q32), and t(14;18)(q32;q21). In addition, a 
      cryptic deletion of the immunoglobulin heavy variable region (IGHV) was revealed. 
      Using 30% as the cutoff for positive CD38 expression, 22 of the 24 cases (92%) 
      were positive for CD38. The present results further confirm that IGH@ 
      rearrangement is not a rare genomic abnormality in B-CLL, and also show both that 
      t(14;19)(q32;q13.2) is the most common cytogenetic change involving IGH@ 
      rearrangement detected by FISH in B-CLL and that IGH@ rearrangement is correlated 
      with CD38 expression. It is appropriate to include an IGH@ probe in the FISH 
      panel for B-CLL diagnosis.
FAU - Lu, Gary
AU  - Lu G
AD  - Department of Hematopathology, Box 350, University of Texas MD Anderson Cancer 
      Center, 1515 Holcombe Blvd., Houston, TX 77030, USA. gglu@mdanderson.org
FAU - Kong, Yue
AU  - Kong Y
FAU - Yue, Changjun
AU  - Yue C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics/immunology
MH  - *Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/*immunology
MH  - Male
MH  - Middle Aged
EDAT- 2009/12/08 06:00
MHDA- 2009/12/18 06:00
CRDT- 2009/12/08 06:00
PHST- 2009/06/25 00:00 [received]
PHST- 2009/08/26 00:00 [revised]
PHST- 2009/08/31 00:00 [accepted]
PHST- 2009/12/08 06:00 [entrez]
PHST- 2009/12/08 06:00 [pubmed]
PHST- 2009/12/18 06:00 [medline]
AID - S0165-4608(09)00490-7 [pii]
AID - 10.1016/j.cancergencyto.2009.08.021 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2010 Jan 1;196(1):56-63. doi: 
      10.1016/j.cancergencyto.2009.08.021.