PMID- 19966531
OWN - NLM
STAT- MEDLINE
DCOM- 20100309
LR  - 20190727
IS  - 1349-3329 (Electronic)
IS  - 0040-8727 (Linking)
VI  - 219
IP  - 4
DP  - 2009 Dec
TI  - Prostatic androgen-repressed message-1 as a regulator of adipocyte 
      differentiation in the mouse.
PG  - 311-7
AB  - Adipocyte differentiation is an important aspect in energy homeostasis. Although 
      the regulation of adipocyte differentiation is relatively well understood, the 
      underlying molecular mechanism remains unclear. In this study, subcutaneous and 
      epididymal adipose tissues were used to study the differential expression of 
      associated genes. We found that the expression level of mouse homologue of rat 
      prostatic androgen-repressed message-1 (mPARM-1) gene was higher in subcutaneous, 
      perirenal and mesenteric adipose tissues than in epididymal adipose tissue. In 
      mouse subcutaneous, perirenal, and mesenteric adipose tissues, the expression 
      level of this gene was higher in adipocytes than in non-adipocyte cells, i.e. 
      stromal-vascular cells. Furthermore, mPARM-1 mRNA expression was up-regulated in 
      subcutaneous, mesenteric, and epididymal adipose tissues of mice fed a high-fat 
      diet compared to those fed a normal-fat diet. Expression level of mPARM-1 mRNA 
      increased in the early stage of the chemically induced adipocyte differentiation, 
      preceding the increase in peroxisome proliferator-activated receptor-gamma 2 
      (PPAR-gamma2) mRNA. Tumor necrosis factor-alpha (TNF-alpha), an inhibitor of 
      adipocyte differentiation, reduced the expression of mPARM-1 mRNA in 
      differentiated 3T3-L1 cells and subsequently down-regulated the expression of 
      adipogenic genes, including PPAR-gamma2, leptin and adipogenin. Moreover, 
      knockdown of mPARM-1 expression with siRNA reduced lipid accumulation and the 
      expression levels of PPAR-gamma2 and adipocyte protein 2 mRNAs, which suggest 
      that the degree of adipocyte differentiation of 3T3-L1 cells has been reduced. 
      These results indicate that mPARM-1 might be involved in the regulation of fat 
      accumulation and adipocyte differentiation.
FAU - Song, Sang-Houn
AU  - Song SH
AD  - Lab of Animal Physiology, Graduate School of Agriculture Science, Tohoku 
      University, Sendai, Japan.
FAU - Hong, Yeon-Hee
AU  - Hong YH
FAU - Sasaki, Shin-ichi
AU  - Sasaki S
FAU - Roh, Sang-Gun
AU  - Roh SG
FAU - Katoh, Kazuo
AU  - Katoh K
LA  - eng
SI  - GENBANK/EF564364
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Tohoku J Exp Med
JT  - The Tohoku journal of experimental medicine
JID - 0417355
RN  - 0 (Androgen-Binding Protein)
RN  - 0 (Dietary Fats)
RN  - 0 (Parm-1 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*cytology/drug effects/*metabolism
MH  - Androgen-Binding Protein/genetics/*metabolism
MH  - Animals
MH  - *Cell Differentiation/drug effects
MH  - Dietary Fats/administration & dosage/pharmacology
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Sequence Analysis, DNA
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2009/12/08 06:00
MHDA- 2010/03/10 06:00
CRDT- 2009/12/08 06:00
PHST- 2009/12/08 06:00 [entrez]
PHST- 2009/12/08 06:00 [pubmed]
PHST- 2010/03/10 06:00 [medline]
AID - JST.JSTAGE/tjem/219.311 [pii]
AID - 10.1620/tjem.219.311 [doi]
PST - ppublish
SO  - Tohoku J Exp Med. 2009 Dec;219(4):311-7. doi: 10.1620/tjem.219.311.