PMID- 19967453
OWN - NLM
STAT- MEDLINE
DCOM- 20110110
LR  - 20211020
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 37
IP  - 7
DP  - 2010 Oct
TI  - FISH is more sensitive than Southern analysis at identifying increased levels of 
      cyclin D1 gene amplified in breast cancer cell lines.
PG  - 3473-80
LID - 10.1007/s11033-009-9939-y [doi]
AB  - Cyclin D1 is involved in regulating the transition of G1 to S-phase in the cell 
      cycle through phosphorylation of the retinoblastoma susceptibility product (pRB). 
      Amplification and overexpression of the cyclin D1 gene (CCND1) have been reported 
      in human breast cancers and are suggested to play important roles in the 
      pathogenesis of the disease process. Although cyclin D1 is potentially an 
      important gene, relatively little is known about the distribution of its 
      amplification in breast cancer cell lines. In this study, a cyclin D1 cosmid 
      probe was isolated and used with fluorescence in situ hybridization (FISH) to 
      identify the gene in chromosomal spreads of 12 breast cancer cell lines. Nine 
      cell lines showed increased gene copy levels of cyclin D1, including Five cell 
      lines had more than six copies of cyclin D1 on sister chromatids and four had 
      more than four copies but less than six copies grouped at the chromosome 11 q13 
      band. Three cell lines had two "normal" chromosome 11 and one and two additional 
      derivative chromosome 11's with three and four 11q13 sites which lacked 
      amplification of cyclin D1 on any of these sites. Using progesterone receptor 
      (PR) gene as an internal control, a 2.0-fold or greater increase in cyclin D1 
      gene signals, was observed in five of the ten cell lines by Southern 
      hybridization, the Amplification level of cyclin D1 varied from 2.3 to 19.6-fold. 
      Three cell lines with low amplification of cyclin D1 showed overexpression of the 
      gene by Northern analysis. Our experiments demonstrated that FISH was more 
      sensitive than Southern blot at demonstrating low levels of gene amplification 
      and, additionally, permitted assessment of the distribution of cyclin D1 gene 
      among chromosomes.
FAU - Dongsong, Nie
AU  - Dongsong N
AD  - Chemistry and Chemical Engineering Department, Hunan Institute of Science and 
      Technology, YueYang, Hunan, 414000, People's Republic of China.
FAU - Zhou, Jian Y
AU  - Zhou JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091205
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (CCND1 protein, human)
RN  - 0 (DNA Probes)
RN  - 0 (RNA, Messenger)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Blotting, Northern
MH  - *Blotting, Southern
MH  - Blotting, Western
MH  - Breast Neoplasms/*genetics
MH  - Cell Line, Tumor
MH  - Chromosomes, Human/genetics
MH  - Cosmids
MH  - Cyclin D1/*genetics
MH  - DNA Probes/metabolism
MH  - Female
MH  - Gene Amplification/*genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Metaphase
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2009/12/08 06:00
MHDA- 2011/01/11 06:00
CRDT- 2009/12/08 06:00
PHST- 2009/05/02 00:00 [received]
PHST- 2009/11/19 00:00 [accepted]
PHST- 2009/12/08 06:00 [entrez]
PHST- 2009/12/08 06:00 [pubmed]
PHST- 2011/01/11 06:00 [medline]
AID - 10.1007/s11033-009-9939-y [doi]
PST - ppublish
SO  - Mol Biol Rep. 2010 Oct;37(7):3473-80. doi: 10.1007/s11033-009-9939-y. Epub 2009 
      Dec 5.