PMID- 19967517
OWN - NLM
STAT- MEDLINE
DCOM- 20100222
LR  - 20091207
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 598
DP  - 2010
TI  - The local lymph node assay and skin sensitization testing.
PG  - 221-31
LID - 10.1007/978-1-60761-401-2_16 [doi]
AB  - The mouse local lymph node assay (LLNA) is a method for the identification and 
      characterization of skin sensitization hazards. In this context the method can be 
      used both to identify contact allergens, and also determine the relative skin 
      sensitizing potency as a basis for derivation of effective risk assessments.The 
      assay is based on measurement of proliferative responses by draining lymph node 
      cells induced following topical exposure of mice to test chemicals. Such 
      responses are known to be causally and quantitatively associated with the 
      acquisition of skin sensitization and therefore provide a relevant marker for 
      characterization of contact allergic potential.The LLNA has been the subject of 
      exhaustive evaluation and validation exercises and has been assigned Organization 
      for Economic Cooperation and Development (OECD) test guideline 429. Herein we 
      describe the conduct and interpretation of the LLNA.
FAU - Kimber, Ian
AU  - Kimber I
FAU - Dearman, Rebecca J
AU  - Dearman RJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Allergens)
SB  - IM
MH  - Allergens/immunology
MH  - Animals
MH  - Dermatitis, Allergic Contact/*immunology
MH  - Dose-Response Relationship, Drug
MH  - Drug Evaluation, Preclinical/*methods
MH  - Drug Hypersensitivity/*immunology
MH  - Female
MH  - *Local Lymph Node Assay
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Risk Assessment
EDAT- 2009/12/08 06:00
MHDA- 2010/02/23 06:00
CRDT- 2009/12/08 06:00
PHST- 2009/12/08 06:00 [entrez]
PHST- 2009/12/08 06:00 [pubmed]
PHST- 2010/02/23 06:00 [medline]
AID - 10.1007/978-1-60761-401-2_16 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;598:221-31. doi: 10.1007/978-1-60761-401-2_16.