PMID- 19967520
OWN - NLM
STAT- MEDLINE
DCOM- 20100222
LR  - 20091207
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 598
DP  - 2010
TI  - Dendritic cells in immunotoxicity testing.
PG  - 259-81
LID - 10.1007/978-1-60761-401-2_19 [doi]
AB  - Dendritic cells (DCs) are now recognized to play the key role in the development 
      of adaptive immunity by promoting activation of naive T cells. Herein, we 
      describe the methodologies to investigate how DCs can be modified by an 
      environmental toxicant and subsequently influence immunity. The prototypic 
      toxicant used as an example for altering DC development and functional influences 
      on T cell development is lead (Pb). It has been reported that the environmental 
      exposure to Pb enhances IgE production in children, which leads to an increase in 
      the incidence of asthma. This effect has been suggested to be due to the 
      preferential enhancement of helper T cell type 2 (Th2) cell responses by Pb. The 
      predominant promotion of Th2 cell development is posited to be due to the altered 
      characteristics of the bone marrow (BM)-DCs from Pb-treated mice (Pb-DCs) when 
      compared to those of the BM-DCs that develop from progenitors in the absence of 
      Pb. The Pb-DCs have a different immunophenotype as well as different cytokine 
      expression after activation. In vitro and in vivo studies confirm that Pb-DCs 
      have the ability to promote antigen-specific T cells to Th2 cells, favoring 
      type-2-related humoral (HI) and cell mediated (CMI) immunity, which may be 
      extracellular signal-regulated kinase (Erk)/mitogen-activated protein (MAP) 
      kinase pathway dependent.
FAU - Gao, Donghong
AU  - Gao D
FAU - Lawrence, David A
AU  - Lawrence DA
LA  - eng
GR  - ES11135/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulins)
RN  - 0 (Xenobiotics)
SB  - IM
MH  - Adaptive Immunity/*immunology
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/cytology/immunology
MH  - Cell Culture Techniques
MH  - Cell Proliferation
MH  - Cytokines/immunology
MH  - Dendritic Cells/cytology/drug effects/*immunology
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Flow Cytometry/methods
MH  - Immunoglobulins/blood/immunology
MH  - Immunologic Tests/*methods
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mice, Transgenic
MH  - Spleen/cytology/immunology
MH  - Toxicity Tests/*methods
MH  - Xenobiotics/immunology/pharmacology
EDAT- 2009/12/08 06:00
MHDA- 2010/02/23 06:00
CRDT- 2009/12/08 06:00
PHST- 2009/12/08 06:00 [entrez]
PHST- 2009/12/08 06:00 [pubmed]
PHST- 2010/02/23 06:00 [medline]
AID - 10.1007/978-1-60761-401-2_19 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;598:259-81. doi: 10.1007/978-1-60761-401-2_19.