PMID- 19969100
OWN - NLM
STAT- MEDLINE
DCOM- 20100413
LR  - 20191210
IS  - 1531-4332 (Electronic)
IS  - 1095-6433 (Linking)
VI  - 155
IP  - 3
DP  - 2010 Mar
TI  - Claudin-15 and -25b expression in the intestinal tract of Atlantic salmon in 
      response to seawater acclimation, smoltification and hormone treatment.
PG  - 361-70
LID - 10.1016/j.cbpa.2009.11.025 [doi]
AB  - In seawater fishes, osmotic homeostasis requires uptake of ions and water in the 
      intestine and these processes are governed by the combined trans- and 
      paracellular pathways. The current study examined mRNA expression of two tight 
      junction proteins (claudin-15 and -25 b) predominantly expressed in the intestine 
      of Atlantic salmon. We examined the response in pyloric caecae, middle and 
      posterior intestine to seawater challenge, during smoltification and after 
      injection with osmoregulatory hormones. Seawater (SW) transfer elevated levels of 
      claudin-15 and -25 b while no change was induced throughout the smolt stage. In 
      freshwater, cortisol and growth hormone inhibited claudin-15 expression in the 
      two anterior segments. Claudin-25 b was elevated in all intestinal segments by 
      growth hormone, while cortisol had an inhibitory effect. In seawater, prolactin 
      and cortisol inhibited claudin expression. The data suggest that claudin 
      expression is involved in the reorganisation of intestinal epithelium and 
      possibly change paracellular permeability during SW acclimation. The lack of 
      preparatory change during smoltification suggests that this process is not 
      completed during smolt development. The effects of the tested hormones cannot 
      explain the sum of changes induced by salinity, which, like the smoltification 
      data, suggests the importance of additional factors and possibly contact with the 
      imbibed SW per se.
CI  - Copyright 2009 Elsevier Inc. All rights reserved.
FAU - Tipsmark, Christian K
AU  - Tipsmark CK
AD  - Institute of Biology, University of Southern Denmark, 5230 Odense M, Denmark. 
      ckt@biology.sdu.dk
FAU - Sorensen, Kenneth J
AU  - Sorensen KJ
FAU - Hulgard, Katrine
AU  - Hulgard K
FAU - Madsen, Steffen S
AU  - Madsen SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091204
PL  - United States
TA  - Comp Biochem Physiol A Mol Integr Physiol
JT  - Comparative biochemistry and physiology. Part A, Molecular & integrative 
      physiology
JID - 9806096
RN  - 0 (Claudins)
RN  - 0 (Hormones)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (claudin 15)
RN  - 9002-72-6 (Growth Hormone)
RN  - 9NEZ333N27 (Sodium)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Acclimatization/*drug effects/genetics
MH  - Animals
MH  - Claudins/*genetics/metabolism
MH  - Female
MH  - *Fresh Water
MH  - Gastrointestinal Tract/drug effects/*metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/drug effects
MH  - Growth Hormone/pharmacology
MH  - Hormones/*pharmacology
MH  - Hydrocortisone/pharmacology
MH  - Injections, Intraperitoneal
MH  - Male
MH  - Membrane Proteins/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Salmo salar/blood/*genetics
MH  - *Seawater
MH  - Sodium/blood
MH  - Sodium-Potassium-Exchanging ATPase/genetics/metabolism
MH  - Time Factors
EDAT- 2009/12/09 06:00
MHDA- 2010/04/14 06:00
CRDT- 2009/12/09 06:00
PHST- 2009/08/27 00:00 [received]
PHST- 2009/11/24 00:00 [revised]
PHST- 2009/11/27 00:00 [accepted]
PHST- 2009/12/09 06:00 [entrez]
PHST- 2009/12/09 06:00 [pubmed]
PHST- 2010/04/14 06:00 [medline]
AID - S1095-6433(09)01182-9 [pii]
AID - 10.1016/j.cbpa.2009.11.025 [doi]
PST - ppublish
SO  - Comp Biochem Physiol A Mol Integr Physiol. 2010 Mar;155(3):361-70. doi: 
      10.1016/j.cbpa.2009.11.025. Epub 2009 Dec 4.