PMID- 19969429
OWN - NLM
STAT- MEDLINE
DCOM- 20101115
LR  - 20121115
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 143
IP  - 2-4
DP  - 2010 Jul 14
TI  - Porcine reproductive and respiratory syndrome virus attachment is mediated by the 
      N-terminal domain of the sialoadhesin receptor.
PG  - 371-8
LID - 10.1016/j.vetmic.2009.11.006 [doi]
AB  - Sialoadhesin (Sn) is an important receptor for viral attachment and 
      internalization of porcine reproductive and respiratory syndrome virus (PRRSV) to 
      porcine alveolar macrophages (PAM). To investigate whether the N-terminal domain 
      of Sn is sufficient and/or necessary for PRRSV attachment, we constructed a 
      series of truncated fragments of porcine Sn and expressed these in the 
      non-permissive PK15 cell line. The first 150 amino acids comprising the entire 
      first domain of the Sn N-terminal region was necessary for PRRSV binding to 
      cells, and the N-terminal domain alone was sufficient for virus attachment. The 
      attachment of PRRSV to PAM cells was inhibited by polyclonal anti-serum against 
      the N-terminal region of porcine Sn in a dose-dependent manner. The present study 
      demonstrates that the first domain at the N-terminus of Sn mediates PRRSV 
      attachment to PAM cells and contributes to better understanding the interaction 
      between PRRSV and its host cells.
CI  - (c) 2009 Elsevier B.V. All rights reserved.
FAU - An, Tong-Qing
AU  - An TQ
AD  - Division of Swine Infectious Diseases, National Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin 150001, China.
FAU - Tian, Zhi-Jun
AU  - Tian ZJ
FAU - He, Yun-Xia
AU  - He YX
FAU - Xiao, Yan
AU  - Xiao Y
FAU - Jiang, Yi-Feng
AU  - Jiang YF
FAU - Peng, Jin-Mei
AU  - Peng JM
FAU - Zhou, Yan-Jun
AU  - Zhou YJ
FAU - Liu, Di
AU  - Liu D
FAU - Tong, Guang-Zhi
AU  - Tong GZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091206
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Core Binding Factors)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 1)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Core Binding Factors
MH  - Gene Deletion
MH  - Macrophages, Alveolar/physiology/*virology
MH  - Membrane Glycoproteins/*physiology
MH  - Models, Molecular
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Protein Binding
MH  - Protein Conformation
MH  - Receptors, Immunologic/*physiology
MH  - Sialic Acid Binding Ig-like Lectin 1
MH  - Swine
MH  - *Virus Attachment
EDAT- 2009/12/09 06:00
MHDA- 2010/11/16 06:00
CRDT- 2009/12/09 06:00
PHST- 2009/04/18 00:00 [received]
PHST- 2009/10/30 00:00 [revised]
PHST- 2009/11/10 00:00 [accepted]
PHST- 2009/12/09 06:00 [entrez]
PHST- 2009/12/09 06:00 [pubmed]
PHST- 2010/11/16 06:00 [medline]
AID - S0378-1135(09)00555-0 [pii]
AID - 10.1016/j.vetmic.2009.11.006 [doi]
PST - ppublish
SO  - Vet Microbiol. 2010 Jul 14;143(2-4):371-8. doi: 10.1016/j.vetmic.2009.11.006. 
      Epub 2009 Dec 6.