PMID- 1997394
OWN - NLM
STAT- MEDLINE
DCOM- 19910404
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 72
IP  - 1
DP  - 1991 Jan
TI  - IgE antibody production in rats against multiple components of 
      excretory-secretory products of the nematode Nippostrongylus brasiliensis.
PG  - 104-8
AB  - Infection with the nematode Nippostrongylus brasiliensis (NB) induces the intense 
      production of specific and non-specific immunoglobulin E (IgE) in rats. In the 
      present study, we analysed NB-derived allergenic substances and the variability 
      of IgE antibody production in response to these allergens in outbred 
      Sprague-Dawley rats. Two kinds of crude allergens were used: the 
      excretory-secretory products (ES) of adult NB, and an extract of homogenized 
      adult worms (AW). ELISA showed that IgE antibody titres to ES were more than five 
      times higher than the titres to AW. In the homologous passive cutaneous 
      anaphylaxis (PCA) reaction using serum from infected rats, as little as 50 
      micrograms of ES had a maximal PCA activity, while even 1 mg of AW still gave a 
      slightly lower PCA titre. Thus, it appeared that ES contained more allergen than 
      AW at the same amount of total proteins. By immunoblot analysis, at least six 
      components were recognized by IgE antibodies from infected animals, and these 
      components were exactly the same in both ES and AW. The results indicated that 
      the allergenic components in ES and AW were the same molecules, and that only 
      those molecules which could be excreted or secreted from living worms were 
      allergenic. Among the array of allergens, 130,000 and 70,000 molecular weight 
      (mw) molecules were commonly recognized by IgE from all serum samples examined, 
      while other components of the allergens were recognized variably by IgE 
      antibodies from individual animals. These findings suggested that individual 
      animals varied considerably in their IgE antibody production to the different 
      constituents of the nematode allergens.
FAU - Yamada, M
AU  - Yamada M
AD  - Department of Medical Zoology, Kyoto Prefectural University of Medicine, Japan.
FAU - Nakazawa, M
AU  - Nakazawa M
FAU - Matsumoto, Y
AU  - Matsumoto Y
FAU - Arizono, N
AU  - Arizono N
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, Helminth)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antibodies, Helminth/*biosynthesis
MH  - Antigens, Helminth/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoblotting
MH  - Immunoglobulin E/*biosynthesis
MH  - Male
MH  - Nematode Infections/*immunology
MH  - Nippostrongylus/*immunology
MH  - Passive Cutaneous Anaphylaxis
MH  - Rats
MH  - Rats, Inbred Strains
PMC - PMC1384344
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
PMCR- 1992/01/01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
PHST- 1992/01/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1991 Jan;72(1):104-8.