PMID- 19995342
OWN - NLM
STAT- MEDLINE
DCOM- 20100806
LR  - 20190911
IS  - 1875-5305 (Electronic)
IS  - 0929-8665 (Linking)
VI  - 17
IP  - 5
DP  - 2010 May
TI  - Kinetics of interaction of HLA-B2705 with natural killer cell immunoglobulin-like 
      receptor 3DS1.
PG  - 547-54
AB  - The recognition of human leukocyte antigen (HLA) molecules by specific receptors 
      is a crucial step in the regulation of natural killer (NK) cell function. Killer 
      cell immunoglobulin-like receptor (KIR) 3DS1 is one of the activating receptors 
      of NK cell and is implicated in slowing disease progression in HIV infection. 
      KIR3DS1 play an important role in the outcome of multiple diseases associated 
      with viral infections. In contrast to the inhibitory receptor, much less is known 
      about the ligands of KIR3DS1. In order to achieve a better understanding of the 
      biology of KIR3DS1 and its ligand systems, it is necessary to identify the 
      ligands of KIR3DS1. In this work, we utilized recombinant HLA-B2705 molecules and 
      DsbA-KIR3DS1 fusion protein to monitor the interaction between HLA-B2705 
      complexes and DsbA-KIR3DS1 using BIAcore 3000 SPR sensor and found that the 
      specific binding between KIR3DS1 and HLA-B2705 existed and the affinity was 
      6.95x10(-6) mol//L. So we concluded that HLA-B2705 is a possible ligand of 
      KIR3DS1.
FAU - Li, Hui
AU  - Li H
AD  - Department of Infectious Disease, Affiliated Hospital, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, Sichuan 610072, China.
FAU - Peng, Shun-Lin
AU  - Peng SL
FAU - Cui, Yu
AU  - Cui Y
FAU - Fu, Qiu-Xia
AU  - Fu QX
FAU - Zhou, Yong
AU  - Zhou Y
FAU - Wang, Quan-Li
AU  - Wang QL
FAU - Zhan, Lin-Sheng
AU  - Zhan LS
FAU - Zhong, Sen
AU  - Zhong S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Protein Pept Lett
JT  - Protein and peptide letters
JID - 9441434
RN  - 0 (HLA-B Antigens)
RN  - 0 (Receptors, KIR3DS1)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (beta 2-Microglobulin)
SB  - IM
MH  - Blotting, Western
MH  - Chromatography, Gel
MH  - Cloning, Molecular
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Escherichia coli/genetics
MH  - HLA-B Antigens/chemistry/genetics/*metabolism
MH  - Humans
MH  - Protein Binding
MH  - Protein Folding
MH  - Receptors, KIR3DS1/chemistry/genetics/*metabolism
MH  - Recombinant Fusion Proteins/chemistry/genetics/*metabolism
MH  - Surface Plasmon Resonance
MH  - beta 2-Microglobulin/genetics/metabolism
EDAT- 2009/12/10 06:00
MHDA- 2010/08/07 06:00
CRDT- 2009/12/10 06:00
PHST- 2009/09/27 00:00 [received]
PHST- 2009/11/15 00:00 [accepted]
PHST- 2009/12/10 06:00 [entrez]
PHST- 2009/12/10 06:00 [pubmed]
PHST- 2010/08/07 06:00 [medline]
AID - 0096 [pii]
AID - 10.2174/092986610791112657 [doi]
PST - ppublish
SO  - Protein Pept Lett. 2010 May;17(5):547-54. doi: 10.2174/092986610791112657.