PMID- 19995935
OWN - NLM
STAT- MEDLINE
DCOM- 20100115
LR  - 20211020
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 187
IP  - 6
DP  - 2009 Dec 14
TI  - Dual roles for the Drosophila PI 4-kinase four wheel drive in localizing Rab11 
      during cytokinesis.
PG  - 847-58
LID - 10.1083/jcb.200908107 [doi]
AB  - Successful completion of cytokinesis relies on addition of new membrane, and 
      requires the recycling endosome regulator Rab11, which localizes to the midzone. 
      Despite the critical role of Rab11 in this process, little is known about the 
      formation and composition of Rab11-containing organelles. Here, we identify the 
      phosphatidylinositol (PI) 4-kinase III beta four wheel drive (Fwd) as a key 
      regulator of Rab11 during cytokinesis in Drosophila melanogaster spermatocytes. 
      We show Fwd is required for synthesis of PI 4-phosphate (PI4P) on Golgi membranes 
      and for formation of PI4P-containing secretory organelles that localize to the 
      midzone. Fwd binds and colocalizes with Rab11 on Golgi membranes, and is required 
      for localization of Rab11 in dividing cells. A kinase-dead version of Fwd also 
      binds Rab11 and partially restores cytokinesis to fwd mutant flies. Moreover, 
      activated Rab11 partially suppresses loss of fwd. Our data suggest Fwd plays 
      catalytic and noncatalytic roles in regulating Rab11 during cytokinesis.
FAU - Polevoy, Gordon
AU  - Polevoy G
AD  - Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, 
      Toronto, Ontario M5G 1L7, Canada.
FAU - Wei, Ho-Chun
AU  - Wei HC
FAU - Wong, Raymond
AU  - Wong R
FAU - Szentpetery, Zsofia
AU  - Szentpetery Z
FAU - Kim, Yeun Ju
AU  - Kim YJ
FAU - Goldbach, Philip
AU  - Goldbach P
FAU - Steinbach, Sarah K
AU  - Steinbach SK
FAU - Balla, Tamas
AU  - Balla T
FAU - Brill, Julie A
AU  - Brill JA
LA  - eng
GR  - 93477/CAPMC/CIHR/Canada
GR  - ImNIH/Intramural NIH HHS/United States
GR  - IG1-93477/CAPMC/CIHR/Canada
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Drosophila Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.1.67 (1-Phosphatidylinositol 4-Kinase)
RN  - EC 3.6.1.- (rab11 protein)
RN  - EC 3.6.5.2 (rab GTP-Binding Proteins)
SB  - IM
MH  - 1-Phosphatidylinositol 4-Kinase/biosynthesis/genetics/*metabolism
MH  - Animals
MH  - COS Cells
MH  - Chlorocebus aethiops
MH  - *Cytokinesis
MH  - Drosophila Proteins/*metabolism
MH  - Drosophila melanogaster/*enzymology/genetics
MH  - Fertility
MH  - Golgi Apparatus/enzymology
MH  - Isoenzymes
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Protein Binding
MH  - Recombinant Fusion Proteins/metabolism
MH  - Secretory Vesicles/enzymology
MH  - Spermatocytes/*enzymology
MH  - Transfection
MH  - Two-Hybrid System Techniques
MH  - rab GTP-Binding Proteins/genetics/*metabolism
PMC - PMC2806325
EDAT- 2009/12/10 06:00
MHDA- 2010/01/16 06:00
PMCR- 2010/06/14
CRDT- 2009/12/10 06:00
PHST- 2009/12/10 06:00 [entrez]
PHST- 2009/12/10 06:00 [pubmed]
PHST- 2010/01/16 06:00 [medline]
PHST- 2010/06/14 00:00 [pmc-release]
AID - jcb.200908107 [pii]
AID - 200908107 [pii]
AID - 10.1083/jcb.200908107 [doi]
PST - ppublish
SO  - J Cell Biol. 2009 Dec 14;187(6):847-58. doi: 10.1083/jcb.200908107.