PMID- 19997642
OWN - NLM
STAT- MEDLINE
DCOM- 20100317
LR  - 20220408
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 4
IP  - 12
DP  - 2009 Dec 4
TI  - Comprehensive analysis of inflammatory immune mediators in vitreoretinal 
      diseases.
PG  - e8158
LID - 10.1371/journal.pone.0008158 [doi]
LID - e8158
AB  - Inflammation affects the formation and the progression of various vitreoretinal 
      diseases. We performed a comprehensive analysis of inflammatory immune mediators 
      in the vitreous fluids from total of 345 patients with diabetic macular edema 
      (DME, n = 92), proliferative diabetic retinopathy (PDR, n = 147), branch retinal 
      vein occlusion (BRVO, n = 30), central retinal vein occlusion (CRVO, n = 13) and 
      rhegmatogenous retinal detachment (RRD, n = 63). As a control, we selected a 
      total of 83 patients with either idiopathic macular hole (MH) or idiopathic 
      epiretinal membrane (ERM) that were free of major pathogenic intraocular changes, 
      such as ischemic retina and proliferative membranes. The concentrations of 20 
      soluble factors (nine cytokines, six chemokines, and five growth factors) were 
      measured simultaneously by multiplex bead analysis system. Out of 20 soluble 
      factors, three factors: interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte 
      chemoattractant protein-1 (MCP-1) were significantly elevated in all groups of 
      vitreoretinal diseases (DME, PDR, BRVO, CRVO, and RRD) compared with control 
      group. According to the correlation analysis in the individual patient's level, 
      these three factors that were simultaneously increased, did not show any 
      independent upregulation in all the examined diseases. Vascular endothelial 
      growth factor (VEGF) was significantly elevated in patients with PDR and CRVO. In 
      PDR patients, the elevation of VEGF was significantly correlated with the three 
      factors: IL-6, IL-8, and MCP-1, while no significant correlation was observed in 
      CRVO patients. In conclusion, multiplex bead system enabled a comprehensive 
      soluble factor analysis in vitreous fluid derived from variety of patients. Major 
      three factors: IL-6, IL-8, and MCP-1 were strongly correlated with each other 
      indicating a common pathway involved in inflammation process in vitreoretinal 
      diseases.
FAU - Yoshimura, Takeru
AU  - Yoshimura T
AD  - Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu 
      University, Fukuoka, Japan.
FAU - Sonoda, Koh-hei
AU  - Sonoda KH
FAU - Sugahara, Mika
AU  - Sugahara M
FAU - Mochizuki, Yasutaka
AU  - Mochizuki Y
FAU - Enaida, Hiroshi
AU  - Enaida H
FAU - Oshima, Yuji
AU  - Oshima Y
FAU - Ueno, Akifumi
AU  - Ueno A
FAU - Hata, Yasuaki
AU  - Hata Y
FAU - Yoshida, Hiroki
AU  - Yoshida H
FAU - Ishibashi, Tatsuro
AU  - Ishibashi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091204
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Aged
MH  - Cytokines/blood
MH  - Diabetic Retinopathy/blood/immunology/pathology
MH  - Female
MH  - Humans
MH  - Inflammation Mediators/blood/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Models, Biological
MH  - Retinal Diseases/blood/*immunology/*pathology
MH  - Retinal Vein Occlusion/blood/immunology/pathology
MH  - Solubility
MH  - Vascular Endothelial Growth Factor A/blood
MH  - Vitreoretinopathy, Proliferative/blood/immunology/pathology
MH  - Vitreous Body/*immunology/*pathology
PMC - PMC2780733
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2009/12/10 06:00
MHDA- 2010/03/18 06:00
PMCR- 2009/12/04
CRDT- 2009/12/10 06:00
PHST- 2009/06/25 00:00 [received]
PHST- 2009/11/11 00:00 [accepted]
PHST- 2009/12/10 06:00 [entrez]
PHST- 2009/12/10 06:00 [pubmed]
PHST- 2010/03/18 06:00 [medline]
PHST- 2009/12/04 00:00 [pmc-release]
AID - 09-PONE-RA-11504R1 [pii]
AID - 10.1371/journal.pone.0008158 [doi]
PST - epublish
SO  - PLoS One. 2009 Dec 4;4(12):e8158. doi: 10.1371/journal.pone.0008158.