PMID- 19998278
OWN - NLM
STAT- MEDLINE
DCOM- 20100504
LR  - 20220321
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 105
IP  - 5
DP  - 2010 Apr 1
TI  - The effect of isolated lignins, obtained from a range of pretreated 
      lignocellulosic substrates, on enzymatic hydrolysis.
PG  - 871-9
LID - 10.1002/bit.22626 [doi]
AB  - The influence of the residual lignin remaining in the cellulosic rich component 
      of pretreated lignocellulosic substrates on subsequent enzymatic hydrolysis was 
      assessed. Twelve lignin preparations were isolated by two isolation methods 
      (protease treated lignin (PTL) and cellulolytic enzymatic lignin (CEL)) from 
      three types of biomass (corn stover, poplar, and lodgepole pine) that had been 
      pretreated by two processes (steam and organosolv pretreatments). Comparative 
      analysis of the isolated lignin showed that the CEL contained lower amounts of 
      carbohydrates and protein than did the PTL and that the isolated lignin from corn 
      stover contained more carbohydrates than did the lignin derived from the poplar 
      and lodgepole pine. The lower yields of acid insoluble lignin (AIL) obtained from 
      the corn stover when using the PTL method indicated that the lignin from the corn 
      stover had a higher hydrophilicity than did the lignin from the poplar and 
      lodgepole pine. The isolated lignin preparations were added to the reaction 
      mixture containing crystalline cellulose (Avicel) and their possible effects on 
      enzymatic hydrolysis were assessed. It was apparent that the lignin isolated from 
      lodgepole pine and steam pretreated poplar decreased the hydrolysis yields of 
      Avicel, whereas the other isolated lignins did not appear to decrease the 
      hydrolysis yields significantly. The hydrolysis yields of the pretreated 
      lignocellulose and those of Avicel containing the PTL showed good correlation, 
      indicating that the nature of the residual lignin obtained after pretreatment 
      significantly influenced hydrolysis.
CI  - (c) 2009 Wiley Periodicals, Inc.
FAU - Nakagame, Seiji
AU  - Nakagame S
AD  - Forest Products Biotechnology, University of British Columbia, 2424 Main Mall, 
      Vancouver, British Columbia, Canada.
FAU - Chandra, Richard P
AU  - Chandra RP
FAU - Saddler, Jack N
AU  - Saddler JN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Carbohydrates)
RN  - 0 (Proteins)
RN  - 11132-73-3 (lignocellulose)
RN  - 9004-34-6 (Cellulose)
RN  - 9005-53-2 (Lignin)
SB  - IM
MH  - *Biomass
MH  - Biotransformation
MH  - Carbohydrates/analysis
MH  - Cellulose/metabolism
MH  - Hydrolysis
MH  - Lignin/chemistry/*metabolism
MH  - Pinus
MH  - Populus
MH  - Proteins/analysis
MH  - Zea mays
EDAT- 2009/12/10 06:00
MHDA- 2010/05/05 06:00
CRDT- 2009/12/10 06:00
PHST- 2009/12/10 06:00 [entrez]
PHST- 2009/12/10 06:00 [pubmed]
PHST- 2010/05/05 06:00 [medline]
AID - 10.1002/bit.22626 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2010 Apr 1;105(5):871-9. doi: 10.1002/bit.22626.