PMID- 20001230
OWN - NLM
STAT- MEDLINE
DCOM- 20100402
LR  - 20211203
IS  - 1029-2403 (Electronic)
IS  - 1026-8022 (Linking)
VI  - 51
IP  - 1
DP  - 2010 Jan
TI  - Genetic changes including gene copy number alterations and their relation to 
      prognosis in childhood acute myeloid leukemia.
PG  - 114-24
LID - 10.3109/10428190903350397 [doi]
AB  - We studied a series of 68 subjects diagnosed with childhood acute myeloid 
      leukemia (AML) using conventional cytogenetics and fluorescence in situ 
      hybridization (FISH), polymerase chain reaction (PCR) to analyze mutations in 
      FLT3 and NPM1 genes, and/or array comparative genomic hybridization (CGH). 
      Cytogenetic/FISH abnormalities were observed in 71% of subjects, FLT3-ITD 
      mutations in 15%, and NPM1 mutations in 13%. The array CGH alterations (average 
      3.6 per case) were observed in 96% of the tested subjects. The most frequent 
      alterations were gains of 8q24.3 and 11p15.5-p15.4 in 16% of the samples. Six 
      genes (AKT1, RUNX1, LTB, SDC1, RUNX1T1, and JAK2) from the imbalanced regions 
      have been reported to be involved in AML, whereas other 30 cancer genes, not 
      previously reported in an AML context, were identified as imbalanced. They 
      probably correspond to non passenger alterations that cooperate with the 
      recurrent translocations. The clinical data and genetic changes were tested to 
      find out the possible association with prognosis. Genomic instability (four or 
      more genomic imbalances) was correlated with poor patient outcome (p = 0.029).
FAU - Armengol, Gemma
AU  - Armengol G
AD  - Unit of Biological Anthropology, Department of Animal Biology, Plant Biology and 
      Ecology, Faculty of Biosciences, Universitat Autonoma de Barcelona, Barcelona, 
      Spain. gemma.armengol@uab.cat
FAU - Canellas, Anna
AU  - Canellas A
FAU - Alvarez, Yolanda
AU  - Alvarez Y
FAU - Bastida, Pilar
AU  - Bastida P
FAU - Toledo, Jose Sanchez De
AU  - Toledo JS
FAU - Perez-Iribarne, Maria Del Mar
AU  - Perez-Iribarne Mdel M
FAU - Camos, Mireia
AU  - Camos M
FAU - Tuset, Esperanza
AU  - Tuset E
FAU - Estella, Jesus
AU  - Estella J
FAU - Coll, Maria Dolores
AU  - Coll MD
FAU - Caballin, Maria Rosa
AU  - Caballin MR
FAU - Knuutila, Sakari
AU  - Knuutila S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
SB  - IM
MH  - Adolescent
MH  - Bone Marrow Cells/cytology
MH  - Child
MH  - Child, Preschool
MH  - Cytogenetics
MH  - Female
MH  - *Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Infant, Newborn
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/diagnosis/*genetics/therapy
MH  - Male
MH  - *Mutation
MH  - Nucleophosmin
MH  - Polymerase Chain Reaction
MH  - Prognosis
MH  - Translocation, Genetic
EDAT- 2009/12/17 06:00
MHDA- 2010/04/03 06:00
CRDT- 2009/12/17 06:00
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/04/03 06:00 [medline]
AID - 10.3109/10428190903350397 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 2010 Jan;51(1):114-24. doi: 10.3109/10428190903350397.