PMID- 20002133
OWN - NLM
STAT- MEDLINE
DCOM- 20101014
LR  - 20211020
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 12
IP  - 3
DP  - 2010 Mar
TI  - Abundances of crenarchaeal amoA genes and transcripts in the Pacific Ocean.
PG  - 679-88
LID - 10.1111/j.1462-2920.2009.02108.x [doi]
AB  - Planktonic Crenarchaea are thought to play a key role in chemolithotrophic 
      ammonia oxidation, a critical step of the marine nitrogen (N) cycle. In this 
      study, we examined the spatial distributions of ammonia-oxidizing Crenarchaea 
      across a large (approximately 5200 km) region of the central Pacific Ocean. 
      Examination of crenarchaeal 16S rRNA, ammonia monooxygenase subunit A (amoA) 
      genes, and amoA transcript abundances provided insight into their spatial 
      distributions and activities. Crenarchaeal gene abundances increased three to 
      four orders of magnitude with depth between the upper ocean waters and dimly lit 
      waters of the mesopelagic zone. The resulting median value of the crenarchaeal 
      amoA: 16S rRNA gene ratio was 1.3, suggesting the majority of Crenarchaea in the 
      epi- and mesopelagic regions of the Pacific Ocean have the metabolic machinery 
      for ammonia oxidation. Crenarchaeal amoA transcript abundances typically 
      increased one to two orders of magnitude in the transitional zone separating the 
      epipelagic waters from the mesopelagic (100-200 m), before decreasing into the 
      interior of the mesopelagic zone. The resulting gene copy normalized transcript 
      abundances revealed elevated amoA expression in the upper ocean waters (0-100 m) 
      where crenarchaeal abundances were low, with transcripts decreasing into the 
      mesopelagic zone as crenarchaeal gene abundances increased. These results suggest 
      ammonia-oxidizing Crenarchaea are active contributors to the N cycle throughout 
      the epi- and mesopelagic waters of the Pacific Ocean.
FAU - Church, Matthew J
AU  - Church MJ
AD  - School of Ocean and Earth Science and Technology, Department of Oceanography, 
      University of Hawaii, Honolulu, HI 96822, USA. mjchurch@hawaii.edu
FAU - Wai, Brenner
AU  - Wai B
FAU - Karl, David M
AU  - Karl DM
FAU - DeLong, Edward F
AU  - DeLong EF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20091125
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Ammonia/metabolism
MH  - Crenarchaeota/*enzymology/*genetics
MH  - Oxidoreductases/*genetics/metabolism
MH  - Pacific Ocean
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seawater/*microbiology
MH  - *Water Microbiology
PMC - PMC2847202
EDAT- 2009/12/17 06:00
MHDA- 2010/10/15 06:00
CRDT- 2009/12/17 06:00
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/10/15 06:00 [medline]
AID - EMI2108 [pii]
AID - 10.1111/j.1462-2920.2009.02108.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2010 Mar;12(3):679-88. doi: 10.1111/j.1462-2920.2009.02108.x. 
      Epub 2009 Nov 25.