PMID- 20004699
OWN - NLM
STAT- MEDLINE
DCOM- 20100902
LR  - 20100531
IS  - 1638-6183 (Electronic)
IS  - 0300-9084 (Linking)
VI  - 92
IP  - 6
DP  - 2010 Jun
TI  - Attenuated Th1 induction by dendritic cells from mice deficient in the 
      leukotriene B4 receptor 1.
PG  - 682-91
LID - 10.1016/j.biochi.2009.12.002 [doi]
AB  - Dendritic cells (DCs) are important antigen-presenting cells that control Th1- 
      and Th2-type immunological reactions by releasing cytokines and interacting 
      directly with T cells. Leukotriene B4 (LTB4), a classical proinflammatory lipid 
      mediator for phagocytes, was recently identified as an important attractant for 
      effector CD4(+) and CD8(+) T cells. However, little information is available on 
      the roles of LTB4 and its receptor BLT1 in DCs. Here we show that functional BLT1 
      expressed in mouse bone marrow-derived DCs (BMDCs) plays important role in 
      initiating Th1-type immune response. Detailed analyses using BMDCs revealed that 
      BLT1-deficient DCs produced less IL-12p70 than WT DCs, leading to attenuated 
      IFN-gamma production in an allogeneic mixed lymphocyte reaction. Adoptive 
      transfer of antigen-loaded BLT1-deficient DCs into naive WT mice induced a 
      weakened Th1- and enhanced Th2-response in vivo compared to WT DCs. 
      BLT1-deficient mice consistently showed much attenuated delayed-type 
      hypersensitivity (DTH), in which Th1-type cellular responses play a key role, and 
      popliteal lymph node cells of BLT1-deficient mice showed reduced production of 
      Th1 cytokines after DTH induction compared to cells from WT mice. Thus, in 
      addition to its role in inflammation, the LTB4-BLT1 axis is important in 
      initiating Th1-type immunological reactions mediated by DCs.
CI  - Copyright 2010 Elsevier Masson SAS. All rights reserved.
FAU - Toda, Akiko
AU  - Toda A
AD  - Department of Biochemistry and Molecular Biology, Faculty of Medicine, The 
      University of Tokyo, Tokyo 113-0033, Japan.
FAU - Terawaki, Kan
AU  - Terawaki K
FAU - Yamazaki, Soh
AU  - Yamazaki S
FAU - Saeki, Kazuko
AU  - Saeki K
FAU - Shimizu, Takao
AU  - Shimizu T
FAU - Yokomizo, Takehiko
AU  - Yokomizo T
LA  - eng
PT  - Journal Article
DEP - 20091223
PL  - France
TA  - Biochimie
JT  - Biochimie
JID - 1264604
RN  - 0 (Ltb4r1 protein, mouse)
RN  - 0 (Receptors, Leukotriene B4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Interferon-gamma/metabolism
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Receptors, Leukotriene B4/*genetics/metabolism
MH  - Th1 Cells/*immunology
EDAT- 2009/12/17 06:00
MHDA- 2010/09/03 06:00
CRDT- 2009/12/17 06:00
PHST- 2009/09/25 00:00 [received]
PHST- 2009/12/02 00:00 [accepted]
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/09/03 06:00 [medline]
AID - S0300-9084(09)00333-2 [pii]
AID - 10.1016/j.biochi.2009.12.002 [doi]
PST - ppublish
SO  - Biochimie. 2010 Jun;92(6):682-91. doi: 10.1016/j.biochi.2009.12.002. Epub 2009 
      Dec 23.