PMID- 20005281
OWN - NLM
STAT- MEDLINE
DCOM- 20100409
LR  - 20131121
IS  - 1879-3185 (Electronic)
IS  - 0300-483X (Linking)
VI  - 270
IP  - 1
DP  - 2010 Mar 30
TI  - Intermittent dosing of G-CSF to ameliorate carbon tetrachloride-induced liver 
      fibrosis in mice.
PG  - 43-8
LID - 10.1016/j.tox.2009.12.002 [doi]
AB  - On the basis of the recent report that granulocyte colony-stimulating factor 
      (G-CSF) administration after rats' partial orthotopic liver transplantation 
      greatly improved survival rate and liver regeneration of partial graft, we here 
      evaluated the effect of intermittent administration of G-CSF on fibrosis 
      formation induced by carbon tetrachloride (CCl(4)). Bone marrow chimeric female 
      C57BL/6 mice were treated with G-CSF at days 1, 7, 14, 21, and 28 after CCl(4) 
      challenge. At day 35 after CCl(4) administration, we found that G-CSF treatment 
      significantly reduced CCl(4)-induced liver damage and collagen deposition. In 
      addition, levels of hepatic hydroxyproline and serum fibrosis markers in mice 
      receiving G-CSF administration after CCl(4) challenge were significantly lower 
      compared to those of control mice. Histological examination suggested that 
      hepatic damage recovery was much better in these G-CSF-treated mice. 
      Immunofluorescence and fluorescence in situ hybridization (FISH) analysis 
      revealed that donor cells engrafted into host liver, had epithelium-like 
      morphology and expressed albumin, although at low frequency. These results 
      suggest that intermittent G-CSF treatment might initiate endogenous hepatic 
      tissue regeneration in response to CCl(4) injury and ameliorate its fibrogenic 
      effects.
CI  - (c) 2009 Elsevier Ireland Ltd. All rights reserved.
FAU - Fang, Baijun
AU  - Fang B
AD  - Department of Haematology, Center of Excellence in Tissue Engineering, Henan 
      Tumor Hospital, Zhengzhou University, 127 Dongming Road, Zhengzhou 450008, China.
FAU - Luo, Suxia
AU  - Luo S
FAU - Song, Yongping
AU  - Song Y
FAU - Li, Ning
AU  - Li N
FAU - Li, Huixiang
AU  - Li H
FAU - Zhao, Robert Chunhua
AU  - Zhao RC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091211
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - RMB44WO89X (Hydroxyproline)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/drug effects
MH  - Carbon Tetrachloride Poisoning/*pathology/*prevention & control
MH  - Cell Transplantation
MH  - Colony-Forming Units Assay
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Granulocyte Colony-Stimulating Factor/*therapeutic use
MH  - Hydroxyproline/metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Liver/metabolism/pathology
MH  - Liver Cirrhosis/*pathology/*prevention & control
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Recombinant Proteins
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2009/12/17 06:00
MHDA- 2010/04/10 06:00
CRDT- 2009/12/17 06:00
PHST- 2009/02/02 00:00 [received]
PHST- 2009/12/03 00:00 [accepted]
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/04/10 06:00 [medline]
AID - S0300-483X(09)00606-4 [pii]
AID - 10.1016/j.tox.2009.12.002 [doi]
PST - ppublish
SO  - Toxicology. 2010 Mar 30;270(1):43-8. doi: 10.1016/j.tox.2009.12.002. Epub 2009 
      Dec 11.