PMID- 20005592
OWN - NLM
STAT- MEDLINE
DCOM- 20100505
LR  - 20131121
IS  - 1879-3460 (Electronic)
IS  - 0168-1605 (Linking)
VI  - 138
IP  - 1-2
DP  - 2010 Mar 31
TI  - Susceptibility to tetracycline and erythromycin of Lactobacillus paracasei 
      strains isolated from traditional Italian fermented foods.
PG  - 151-6
LID - 10.1016/j.ijfoodmicro.2009.11.018 [doi]
AB  - The aim of this study was to evaluate the susceptibility of 197 isolates of 
      Lactobacillus paracasei, isolated from Italian fermented products coming from 
      different geographical areas, to tetracycline and erythromycin, two 
      antimicrobials widely used in clinical and animal therapy. Isolation media were 
      supplemented with antibiotics according to the microbiological breakpoints (BPs) 
      defined by European Food Safety Authority (EFSA). Isolates were identified at the 
      species level and were typed by rep-PCR using the (GTG)(5) primer. A total of 121 
      genotypically different strains were detected and their phenotypic antimicrobial 
      resistance to tetracycline and erythromycin was determined as the minimum 
      inhibitory concentration (MIC) using the broth microdilution method. The presence 
      of the genes ermB, ermC and tetL, tetM, tetS, tetW, in the phenotypically 
      resistant isolates was investigated by PCR. Tetracycline induction of tetM 
      expression on representative resistant strains, grown in medium either lacking or 
      containing the antibiotic, was also analyzed by RT-PCR. Among the 121 tested 
      strains, 77.7% were susceptible to tetracycline (MIC<or=4 microg/ml; BP=4 
      microg/ml) and 94.2% to erythromycin (MIC<or=1 microg/ml; BP=1 microg/ml). 
      Overall, 27 strains appeared to be resistant to tetracycline (16<or=MIC<or=128 
      microg/ml ) (Tet(R)), and 7 of them were also resistant to erythromycin 
      (MIC>or=1024 microg/ml) (Erm(R)). The tetM and ermB genes were the most 
      frequently detected in the Tet(R) and/or Erm(R) strains. The tetM expression was 
      induced by antibiotic addition to the growth medium. Our study confirmed that L. 
      paracasei is quite sensitive to tetracycline and erythromycin, but the high level 
      of resistance of Erm(R) strains suggested that acquired resistance took place. 
      Further investigations are required to analyze whether the genes identified in L. 
      paracasei isolates might be horizontally transferred to other species. Since 
      "commensal" bacteria, which L. paracasei belongs to, may play an active role in 
      the spreading of antibiotic resistance, a series of measures inspired from a 
      principle of precaution should be taken before they are used as commercial 
      starters or probiotic cultures in food products, complemented by a more prudent 
      use of antibiotics in agriculture, veterinary, and human medicine.
CI  - Copyright 2009 Elsevier B.V. All rights reserved.
FAU - Comunian, Roberta
AU  - Comunian R
AD  - AGRIS Sardegna, Dipartimento per la Ricerca nelle Produzioni Animali, Olmedo 
      (SS), Italy. rcomunian@agrisricerca.it
FAU - Daga, Elisabetta
AU  - Daga E
FAU - Dupre, Ilaria
AU  - Dupre I
FAU - Paba, Antonio
AU  - Paba A
FAU - Devirgiliis, Chiara
AU  - Devirgiliis C
FAU - Piccioni, Valeria
AU  - Piccioni V
FAU - Perozzi, Giuditta
AU  - Perozzi G
FAU - Zonenschain, Daniela
AU  - Zonenschain D
FAU - Rebecchi, Annalisa
AU  - Rebecchi A
FAU - Morelli, Lorenzo
AU  - Morelli L
FAU - De Lorentiis, Angela
AU  - De Lorentiis A
FAU - Giraffa, Giorgio
AU  - Giraffa G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091203
PL  - Netherlands
TA  - Int J Food Microbiol
JT  - International journal of food microbiology
JID - 8412849
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (DNA, Bacterial)
RN  - 63937KV33D (Erythromycin)
RN  - F8VB5M810T (Tetracycline)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Colony Count, Microbial
MH  - Consumer Product Safety
MH  - DNA, Bacterial/analysis
MH  - Dose-Response Relationship, Drug
MH  - Drug Resistance, Bacterial/*genetics
MH  - Erythromycin/pharmacology
MH  - Fermentation
MH  - Food Contamination/*analysis
MH  - Food Microbiology
MH  - *Gene Transfer, Horizontal
MH  - Humans
MH  - Italy
MH  - Lactobacillus/*drug effects/genetics
MH  - Microbial Sensitivity Tests
MH  - Tetracycline/pharmacology
EDAT- 2009/12/17 06:00
MHDA- 2010/05/06 06:00
CRDT- 2009/12/17 06:00
PHST- 2009/10/05 00:00 [received]
PHST- 2009/11/09 00:00 [revised]
PHST- 2009/11/19 00:00 [accepted]
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/05/06 06:00 [medline]
AID - S0168-1605(09)00612-6 [pii]
AID - 10.1016/j.ijfoodmicro.2009.11.018 [doi]
PST - ppublish
SO  - Int J Food Microbiol. 2010 Mar 31;138(1-2):151-6. doi: 
      10.1016/j.ijfoodmicro.2009.11.018. Epub 2009 Dec 3.