PMID- 20005873
OWN - NLM
STAT- MEDLINE
DCOM- 20100216
LR  - 20131121
IS  - 1090-2430 (Electronic)
IS  - 0014-4886 (Linking)
VI  - 221
IP  - 2
DP  - 2010 Feb
TI  - Bone morphogenetic proteins mediate cellular response and, together with Noggin, 
      regulate astrocyte differentiation after spinal cord injury.
PG  - 353-66
LID - 10.1016/j.expneurol.2009.12.003 [doi]
AB  - Bone morphogenetic proteins (BMPs) play a critical role in regulating cell fate 
      determination during central nervous system (CNS) development. In light of recent 
      findings that BMP-2/4/7 expressions are upregulated after spinal cord injury, we 
      hypothesized that the BMP signaling pathway is important in regulating cellular 
      composition in the injured spinal cord. We found that BMP expressions were 
      upregulated in neural stem cells (NSCs), neurons, oligodendrocytes and 
      microglia/macrophages. Increased expression levels of pSmad1/5/8 (downstream 
      molecules of BMP) were detected in neurons, NSCs, astrocytes, oligodendrocytes 
      and oligodendroglial progenitor cells (OPCs). Active astrocytes which form the 
      astroglial scar were probably derived from NSCs, OPCs and resident astrocytes. 
      Since quiescent NSCs in the normal adult spinal cord will proliferate and 
      differentiate actively into neural cells after traumatic injury, we proposed that 
      BMPs can regulate cellular components by controlling NSC differentiation. 
      Neurosphere culture from adult mouse spinal cord showed that BMP-4 promoted 
      astrocyte differentiation from NSCs while suppressing production of neurons and 
      oligodendrocytes. Conversely, inhibition of BMP-4 by Noggin notably decreased the 
      ratio of astrocyte to neuron numbers. However, intrathecal administration of 
      Noggin in the injured spinal cord failed to attenuate glial fibrillar acidic 
      protein (GFAP) expression even though it effectively reduced pSmad expression. 
      Noggin treatment did not block phosphorylation of Stat3 and the induction of GFAP 
      in the injured spinal cord, suggesting that in addition to the BMP/Smad pathway, 
      the JAK/STAT pathway may also be involved in the regulation of GFAP expression 
      after spinal cord injury.
CI  - Copyright 2009 Elsevier Inc. All rights reserved.
FAU - Xiao, Qi
AU  - Xiao Q
AD  - Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong 
      Kong, Pokfulam, Hong Kong SAR, China.
FAU - Du, Yang
AU  - Du Y
FAU - Wu, Wutian
AU  - Wu W
FAU - Yip, Henry K
AU  - Yip HK
LA  - eng
PT  - Journal Article
DEP - 20091211
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Myelin Basic Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 148294-77-3 (noggin protein)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Astrocytes/*drug effects
MH  - Bone Morphogenetic Proteins/*pharmacology/*therapeutic use
MH  - Bromodeoxyuridine/metabolism
MH  - Carrier Proteins/*pharmacology/*therapeutic use
MH  - Cell Differentiation/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myelin Basic Protein/genetics/metabolism
MH  - Nerve Tissue Proteins/genetics/metabolism
MH  - Neurons
MH  - Nuclear Proteins/genetics/metabolism
MH  - Oligodendroglia/drug effects
MH  - RNA, Messenger/metabolism
MH  - Signal Transduction/drug effects/physiology
MH  - *Spinal Cord Injuries/drug therapy/pathology/physiopathology
MH  - Stem Cells/drug effects
MH  - Time Factors
EDAT- 2009/12/17 06:00
MHDA- 2010/02/17 06:00
CRDT- 2009/12/17 06:00
PHST- 2009/08/04 00:00 [received]
PHST- 2009/10/31 00:00 [revised]
PHST- 2009/12/01 00:00 [accepted]
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/02/17 06:00 [medline]
AID - S0014-4886(09)00481-6 [pii]
AID - 10.1016/j.expneurol.2009.12.003 [doi]
PST - ppublish
SO  - Exp Neurol. 2010 Feb;221(2):353-66. doi: 10.1016/j.expneurol.2009.12.003. Epub 
      2009 Dec 11.