PMID- 20005922
OWN - NLM
STAT- MEDLINE
DCOM- 20100504
LR  - 20191210
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 166
IP  - 1
DP  - 2010 Mar 10
TI  - Caloric restriction provided after global ischemia does not reduce hippocampal 
      cornu ammonis injury or improve functional recovery.
PG  - 263-70
LID - 10.1016/j.neuroscience.2009.11.076 [doi]
AB  - Since caloric restriction (CR) can modify multiple pathways central to the 
      ischemic cascade and enhance neuroplasticity mechanisms, we hypothesized that CR 
      should exert protective effects following brain ischemia. Previous studies have 
      suggested benefit when CR was administered prior to ischemia. We investigated 
      whether prolonged CR beginning after global ischemia would result in lasting 
      protection as assessed by performance in the open field, as a measure of 
      functional outcome, and hippocampal CA1 neuronal counts. Adult, male Mongolian 
      gerbils were subjected to 5 min bilateral carotid artery occlusion (ISCH) or sham 
      surgery (SHAM) with tympanic temperature maintained at 36.5+/-0.2 degrees C 
      during the intra-ischemic period. After screening out gerbils with incomplete 
      ischemia, each of the two surgical groups were randomly assigned to control diet 
      (CON) or 30% CR for the duration of the study (60 d). Gerbils were tested in the 
      open field on d3, 7, 10, 30 and 60. ISCH-CON animals showed a significantly 
      higher level of activity in the open field (impaired habituation) compared to 
      SHAM-CON gerbils on all test days (P<0.001). Open field activity was 
      significantly lower in the ISCH-CR group than in ISCH-CON gerbils only on d7 
      (P=0.024). Open field activity of the SHAM-CR gerbils showed a trend to increase 
      relative to that of SHAM-CON gerbils during the last 30 d of the study (P=0.055 
      on d60), raising the question of suitability of the open field test for long-term 
      studies of CR and ischemia. Brain sections obtained at d60 were stained with 
      hematoxylin and eosin. Hippocampal CA1 neuron counts were significantly reduced 
      by ischemia (P<0.001), and there was no sparing effect of CR. Our findings 
      suggest that prolonged 30% CR administered beginning after global ischemia cannot 
      diminish brain injury or enhance long-term recovery.
CI  - Copyright 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - McEwen, B R
AU  - McEwen BR
AD  - College of Pharmacy and Nutrition and Cameco Multiple Sclerosis Neuroscience 
      Research Center, University of Saskatchewan, 110 Science Place, Saskatoon, SK, 
      Canada.
FAU - Paterson, P G
AU  - Paterson PG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091228
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
SB  - IM
MH  - Animals
MH  - Brain Ischemia/*metabolism/physiopathology/*therapy
MH  - CA1 Region, Hippocampal/*metabolism/pathology/physiopathology
MH  - Caloric Restriction/*methods
MH  - Cell Count
MH  - Cognition Disorders/metabolism/pathology/physiopathology
MH  - Disease Models, Animal
MH  - Eating/physiology
MH  - Energy Metabolism/physiology
MH  - Food Deprivation/*physiology
MH  - Food, Formulated
MH  - Gerbillinae
MH  - Learning Disabilities/metabolism/pathology/physiopathology
MH  - Male
MH  - Nerve Degeneration/physiopathology/prevention & control/*therapy
MH  - Neurons/metabolism/pathology
MH  - Recovery of Function/physiology
EDAT- 2009/12/17 06:00
MHDA- 2010/05/05 06:00
CRDT- 2009/12/17 06:00
PHST- 2009/07/21 00:00 [received]
PHST- 2009/11/15 00:00 [revised]
PHST- 2009/11/28 00:00 [accepted]
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/05/05 06:00 [medline]
AID - S0306-4522(09)01988-5 [pii]
AID - 10.1016/j.neuroscience.2009.11.076 [doi]
PST - ppublish
SO  - Neuroscience. 2010 Mar 10;166(1):263-70. doi: 10.1016/j.neuroscience.2009.11.076. 
      Epub 2009 Dec 28.