PMID- 20006573
OWN - NLM
STAT- MEDLINE
DCOM- 20100308
LR  - 20171116
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 391
IP  - 2
DP  - 2010 Jan 8
TI  - Tumor Necrosis Factor alpha (TNFalpha) regulates CD40 expression through SMAR1 
      phosphorylation.
PG  - 1255-61
LID - 10.1016/j.bbrc.2009.12.055 [doi]
AB  - CD40 plays an important role in mediating inflammatory response and is mainly 
      induced by JAK/STAT phosphorylation cascade. TNFalpha is the key cytokine that 
      activates CD40 during inflammation and tumorigenesis. We have earlier shown that 
      SMAR1 can repress the transcription of Cyclin D1 promoter by forming a HDAC1 
      dependent repressor complex. In this study, we show that SMAR1 regulates the 
      transcription of NF-kappaB target gene CD40. SMAR1 recruits HDAC1 and forms a 
      repressor complex on CD40 promoter and keeps its basal transcription in check. 
      Further, we show that TNFalpha stimulation induces SMAR1 phosphorylation at 
      Ser-347 and promotes its cytoplasmic translocation, thus releasing its negative 
      effect. Concomitantly, TNFalpha induced phosphorylation of STAT1 at Tyr-701 by 
      JAK1 facilitates its nuclear translocation and activation of CD40 through p300 
      recruitment and core Histone-3 acetylation. Thus, TNFalpha mediated regulation of 
      CD40 expression occurs by dual phosphorylation of SMAR1 and STAT1.
CI  - Copyright 2009 Elsevier Inc. All rights reserved.
FAU - Singh, Kamini
AU  - Singh K
AD  - National Centre for Cell Science, Pune University Campus, Ganeshkhind, Pune 411 
      007, Maharashtra, India.
FAU - Sinha, Surajit
AU  - Sinha S
FAU - Malonia, Sunil Kumar
AU  - Malonia SK
FAU - Chattopadhyay, Samit
AU  - Chattopadhyay S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091216
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Banp protein, mouse)
RN  - 0 (CD40 Antigens)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (STAT1 Transcription Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.10.2 (Janus Kinases)
SB  - IM
MH  - Animals
MH  - CD40 Antigens/*genetics
MH  - Cell Cycle Proteins/*metabolism
MH  - Cell Line
MH  - Cytoplasm/metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - *Gene Expression Regulation
MH  - Janus Kinases/metabolism
MH  - Mice
MH  - Nuclear Proteins/*metabolism
MH  - Phosphorylation
MH  - Promoter Regions, Genetic
MH  - Protein Transport
MH  - STAT1 Transcription Factor/metabolism
MH  - Serine/metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism/pharmacology
EDAT- 2009/12/17 06:00
MHDA- 2010/03/10 06:00
CRDT- 2009/12/17 06:00
PHST- 2009/11/19 00:00 [received]
PHST- 2009/12/10 00:00 [accepted]
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/03/10 06:00 [medline]
AID - S0006-291X(09)02428-0 [pii]
AID - 10.1016/j.bbrc.2009.12.055 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2010 Jan 8;391(2):1255-61. doi: 
      10.1016/j.bbrc.2009.12.055. Epub 2009 Dec 16.