PMID- 20006597
OWN - NLM
STAT- MEDLINE
DCOM- 20100330
LR  - 20221207
IS  - 1879-0712 (Electronic)
IS  - 0014-2999 (Linking)
VI  - 629
IP  - 1-3
DP  - 2010 Mar 10
TI  - Antagonists and substrates differentially regulate serotonin transporter cell 
      surface expression in serotonergic neurons.
PG  - 63-7
LID - 10.1016/j.ejphar.2009.12.010 [doi]
AB  - The serotonin transporter (SERT) terminates serotonergic neurotransmission by the 
      rapid removal of serotonin (5-hydroxytryptamine, 5-HT) from the extracellular 
      space back into serotonergic neurons. SERT therefore controls the concentration 
      of extracellular 5-HT, and thus one mechanism to regulate the efficacy of 
      serotonergic neurotransmission is via modulation of the density of SERT molecules 
      on the cell membrane. We have studied the effects of prolonged exposure to 
      various selective serotonin re-uptake inhibitors (SSRIs), as well as cocaine and 
      the transport substrates 5-HT and 3,4-methylenedioxy-methamphetamine (MDMA), on 
      SERT cell surface expression in cultured serotonergic neurons. This was achieved 
      via quantification of the amount of cell surface-expressed SERT molecules using 
      antibody detection combined with confocal laser scanning microscopy. Our results 
      show that exposure to the SSRIs citalopram, fluoxetine, sertraline and paroxetine 
      all induced SERT internalization, but with different efficacies. The substrates 
      5-HT and MDMA also induced SERT internalization, while cocaine elevated SERT cell 
      surface expression.
CI  - Copyright (c) 2009 Elsevier B.V. All rights reserved.
FAU - Kittler, Katharina
AU  - Kittler K
AD  - Biochemical Laboratory, Central Institute of Mental Health, J5, 68159 Mannheim, 
      Germany.
FAU - Lau, Thorsten
AU  - Lau T
FAU - Schloss, Patrick
AU  - Schloss P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091216
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Serotonin Plasma Membrane Transport Proteins)
RN  - 0 (Serotonin Uptake Inhibitors)
RN  - 0DHU5B8D6V (Citalopram)
RN  - 333DO1RDJY (Serotonin)
RN  - I5Y540LHVR (Cocaine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Citalopram/pharmacology
MH  - Cocaine/pharmacology
MH  - Gene Expression Regulation/*drug effects
MH  - N-Methyl-3,4-methylenedioxyamphetamine/metabolism/pharmacology
MH  - Neurons/cytology/*drug effects/*metabolism
MH  - Protein Transport/drug effects
MH  - Serotonin/*metabolism/pharmacology
MH  - Serotonin Plasma Membrane Transport Proteins/*metabolism
MH  - Selective Serotonin Reuptake Inhibitors/*pharmacology
EDAT- 2009/12/17 06:00
MHDA- 2010/03/31 06:00
CRDT- 2009/12/17 06:00
PHST- 2009/10/19 00:00 [received]
PHST- 2009/11/19 00:00 [revised]
PHST- 2009/12/07 00:00 [accepted]
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/03/31 06:00 [medline]
AID - S0014-2999(09)01131-5 [pii]
AID - 10.1016/j.ejphar.2009.12.010 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2010 Mar 10;629(1-3):63-7. doi: 10.1016/j.ejphar.2009.12.010. 
      Epub 2009 Dec 16.