PMID- 20006971
OWN - NLM
STAT- MEDLINE
DCOM- 20100504
LR  - 20111117
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 166
IP  - 1
DP  - 2010 Mar 10
TI  - Neuron-restrictive silencer factor causes epigenetic silencing of Kv4.3 gene 
      after peripheral nerve injury.
PG  - 1-4
LID - 10.1016/j.neuroscience.2009.12.021 [doi]
AB  - Peripheral nerve injury causes a variety of alterations in pain-related gene 
      expression in primary afferent, which underlie the neuronal plasticity in 
      neuropathic pain. One of the characteristic alterations is a long-lasting 
      downregulation of voltage-gated potassium (K(v)) channel, including K(v)4.3, in 
      the dorsal root ganglion (DRG). The present study showed that nerve injury 
      reduces the messenger RNA (mRNA) expression level of K(v)4.3 gene, which contains 
      a conserved neuron-restrictive silencer element (NRSE), a binding site for 
      neuron-restrictive silencer factor (NRSF). Moreover, we found that injury causes 
      an increase in direct NRSF binding to K(v)4.3-NRSE in the DRG, using chromatin 
      immunoprecipitation (ChIP) assay. ChIP assay further revealed that acetylation of 
      histone H4, but not H3, at K(v)4.3-NRSE is markedly reduced at day 7 post-injury. 
      Finally, the injury-induced K(v)4.3 downregulation was significantly blocked by 
      antisense-knockdown of NRSF. Taken together, these data suggest that nerve injury 
      causes an epigenetic silencing of K(v)4.3 gene mediated through transcriptional 
      suppressor NRSF in the DRG.
CI  - Copyright 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Uchida, H
AU  - Uchida H
AD  - Division of Molecular Pharmacology and Neuroscience, Nagasaki University Graduate 
      School of Biomedical Sciences, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
FAU - Sasaki, K
AU  - Sasaki K
FAU - Ma, L
AU  - Ma L
FAU - Ueda, H
AU  - Ueda H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091213
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Histones)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (RE1-silencing transcription factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
RN  - 0 (Shal Potassium Channels)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Down-Regulation/physiology
MH  - Epigenesis, Genetic/*physiology
MH  - Ganglia, Spinal/metabolism/physiopathology
MH  - Gene Expression Regulation/physiology
MH  - Gene Silencing/*physiology
MH  - Histones/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neuralgia/genetics/metabolism/physiopathology
MH  - Oligonucleotides, Antisense/pharmacology
MH  - Peripheral Nerve Injuries
MH  - Peripheral Nerves/metabolism/physiopathology
MH  - Peripheral Nervous System Diseases/*genetics/metabolism/physiopathology
MH  - Protein Binding/genetics
MH  - RNA, Messenger/metabolism
MH  - Repressor Proteins/*genetics/metabolism
MH  - Sensory Receptor Cells/metabolism
MH  - Shal Potassium Channels/*genetics/metabolism
MH  - Silencer Elements, Transcriptional/genetics
EDAT- 2009/12/17 06:00
MHDA- 2010/05/05 06:00
CRDT- 2009/12/17 06:00
PHST- 2009/11/13 00:00 [received]
PHST- 2009/12/04 00:00 [revised]
PHST- 2009/12/07 00:00 [accepted]
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/05/05 06:00 [medline]
AID - S0306-4522(09)02056-9 [pii]
AID - 10.1016/j.neuroscience.2009.12.021 [doi]
PST - ppublish
SO  - Neuroscience. 2010 Mar 10;166(1):1-4. doi: 10.1016/j.neuroscience.2009.12.021. 
      Epub 2009 Dec 13.