PMID- 20007837
OWN - NLM
STAT- MEDLINE
DCOM- 20100426
LR  - 20211020
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 51
IP  - 4
DP  - 2010 Apr
TI  - Epifluorescence intravital microscopy of murine corneal dendritic cells.
PG  - 2101-8
LID - 10.1167/iovs.08-2213 [doi]
AB  - Purpose. Dendritic cells (DCs) are antigen-presenting cells vital for initiating 
      immune responses. In this study the authors examined the in vivo migratory 
      capability of resident corneal DCs to various stimuli. Methods. The authors used 
      mice expressing enhanced yellow fluorescent protein (eYFP) under control of the 
      CD11c promoter to visualize corneal DCs. To assess the distribution and mobility 
      of DCs, normal corneas were imaged in vivo and ex vivo with fluorescence 
      microscopy. Intravital microscopy was used to examine the responses of resident 
      central and peripheral corneal DCs to silver nitrate injury, lipopolysaccharide, 
      microspheres, and tumor necrosis factor (TNF-alpha). In some experiments, 
      TNF-alpha injection was used to first induce centripetal migration of DCs to the 
      central cornea, which was subsequently reinjected with microspheres. Results. In 
      normal corneas, DCs were sparsely distributed centrally and were denser in the 
      periphery, with epithelial-level DCs extending into the epithelium. 
      Videomicroscopy showed that though cell processes were in continuous movement, 
      cells generally did not migrate. Within the first 6 hours after stimulation, 
      neither central nor peripheral corneal DCs exhibited significant lateral 
      migration, but central corneal DCs assumed extreme morphologic changes. An 
      increased number of DCs in the TNF-alpha-stimulated central cornea were 
      responsive to subsequent microsphere injection by adopting a migratory behavior, 
      but not with increased speed. Conclusions. In vivo imaging reveals minimal 
      lateral migration of corneal DCs after various stimuli. In contrast, DCs within 
      the central cornea after initial TNF-alpha injection are more likely to respond 
      to a secondary insult with lateral migration.
FAU - Lee, Ellen J
AU  - Lee EJ
AD  - Departments of Ophthalmology, Cell and Developmental Biology, Oregon Health and 
      Science University, Portland, Oregon, USA. leee@ohsu.edu
FAU - Rosenbaum, James T
AU  - Rosenbaum JT
FAU - Planck, Stephen R
AU  - Planck SR
LA  - eng
GR  - K08 EY015448/EY/NEI NIH HHS/United States
GR  - R01 EY013093/EY/NEI NIH HHS/United States
GR  - EY13093/EY/NEI NIH HHS/United States
GR  - EY015448/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20091210
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 95IT3W8JZE (Silver Nitrate)
SB  - IM
MH  - Animals
MH  - Cell Movement/drug effects/physiology
MH  - Cornea/*cytology/drug effects
MH  - Dendritic Cells/*cytology
MH  - Fluorescent Dyes
MH  - Lipopolysaccharides/toxicity
MH  - Luminescent Proteins/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Fluorescence
MH  - Silver Nitrate/toxicity
MH  - Tumor Necrosis Factor-alpha/toxicity
PMC - PMC2868401
EDAT- 2009/12/17 06:00
MHDA- 2010/04/27 06:00
PMCR- 2010/10/01
CRDT- 2009/12/17 06:00
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/04/27 06:00 [medline]
PHST- 2010/10/01 00:00 [pmc-release]
AID - iovs.08-2213 [pii]
AID - 08-2213 [pii]
AID - 10.1167/iovs.08-2213 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2010 Apr;51(4):2101-8. doi: 10.1167/iovs.08-2213. Epub 
      2009 Dec 10.