PMID- 20008070
OWN - NLM
STAT- MEDLINE
DCOM- 20100216
LR  - 20211020
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Print)
IS  - 0021-9193 (Linking)
VI  - 192
IP  - 4
DP  - 2010 Feb
TI  - The cyclic nucleotide monophosphate domain of Xanthomonas campestris global 
      regulator Clp defines a new class of cyclic di-GMP effectors.
PG  - 1020-9
LID - 10.1128/JB.01253-09 [doi]
AB  - The widely conserved second messenger cyclic diguanosine monophosphate (c-di-GMP) 
      plays a key role in quorum-sensing (QS)-dependent production of virulence factors 
      in Xanthomonas campestris pv. campestris. The detection of QS diffusible signal 
      factor (DSF) by the sensor RpfC leads to the activation of response regulator 
      RpfG, which activates virulence gene expression by degrading c-di-GMP. Here, we 
      show that a global regulator in the X. campestris pv. campestris QS regulatory 
      pathway, Clp, is a c-di-GMP effector. c-di-GMP specifically binds to Clp with 
      high affinity and induces allosteric conformational changes that abolish the 
      interaction between Clp and its target gene promoter. Clp is similar to the 
      cyclic AMP (cAMP) binding proteins Crp and Vfr and contains a conserved cyclic 
      nucleotide monophosphate (cNMP) binding domain. Using site-directed mutagenesis, 
      we found that the cNMP binding domain of Clp contains a glutamic acid residue 
      (E99) that is essential for c-di-GMP binding. Substituting the residue with 
      serine (E99S) resulted in decreased sensitivity to changes in the intracellular 
      c-di-GMP level and attenuated bacterial virulence. These data establish the 
      direct role of Clp in the response to fluctuating c-di-GMP levels and depict a 
      novel mechanism by which QS links the second messenger with the X. campestris pv. 
      campestris virulence regulon.
FAU - Tao, Fei
AU  - Tao F
AD  - Institute of Molecular and Cell Biology, Singapore, Singapore.
FAU - He, Ya-Wen
AU  - He YW
FAU - Wu, Dong-Hui
AU  - Wu DH
FAU - Swarup, Sanjay
AU  - Swarup S
FAU - Zhang, Lian-Hui
AU  - Zhang LH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091211
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - 0 (Bacterial Proteins)
RN  - 0 (Clp protein, Xanthomonas campestris)
RN  - 0 (Transcription Factors)
RN  - 0 (Virulence Factors)
RN  - 61093-23-0 (bis(3',5')-cyclic diguanylic acid)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Allosteric Regulation
MH  - Amino Acid Substitution
MH  - Bacterial Proteins/chemistry/*metabolism
MH  - Binding Sites/genetics
MH  - Cyclic GMP/*analogs & derivatives/metabolism
MH  - Mutagenesis, Site-Directed
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - *Quorum Sensing
MH  - Transcription Factors/chemistry/*metabolism
MH  - Virulence
MH  - Virulence Factors/chemistry/genetics/*metabolism
MH  - Xanthomonas campestris/chemistry/*pathogenicity
PMC - PMC2812978
EDAT- 2009/12/17 06:00
MHDA- 2010/02/17 06:00
PMCR- 2009/12/11
CRDT- 2009/12/17 06:00
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/02/17 06:00 [medline]
PHST- 2009/12/11 00:00 [pmc-release]
AID - JB.01253-09 [pii]
AID - 1253-09 [pii]
AID - 10.1128/JB.01253-09 [doi]
PST - ppublish
SO  - J Bacteriol. 2010 Feb;192(4):1020-9. doi: 10.1128/JB.01253-09. Epub 2009 Dec 11.