PMID- 20009881
OWN - NLM
STAT- MEDLINE
DCOM- 20100302
LR  - 20221207
IS  - 1525-1438 (Electronic)
IS  - 1048-891X (Linking)
VI  - 19
IP  - 8
DP  - 2009 Nov
TI  - Gain of the human telomerase RNA gene TERC at 3q26 is strongly associated with 
      cervical intraepithelial neoplasia and carcinoma.
PG  - 1303-6
LID - 10.1111/IGC.0b013e3181b62ea5 [doi]
AB  - This study investigated the gain of the human telomerase RNA gene TERC at 3q26 in 
      patients with uterine cervix disease in the southern part of China and assessed 
      the relationship between TERC gain and cervical pathological findings. One 
      hundred ten cervical specimens, which were collected from patients with various 
      kinds of uterine cervix disease that was subsequently diagnosed as chronic 
      cervicitis and with examination results negative for intraepithelial lesion or 
      malignancy (NILM, n = 23), mild dysplasia (cervical intraepithelial neoplasia 
      type 1 [CIN1], n = 37), moderate dysplasia (CIN2, n = 12), severe dysplasia 
      (CIN3, n = 10), and squamous cell carcinoma (SCA, n = 28) confirmed by histologic 
      diagnosis, were analyzed for the proportion of abnormal cells with TERC gain 
      using a commercially available 2-color fluorescence in situ hybridization (FISH) 
      probe. The cases with a higher proportion of abnormal cells than the threshold 
      evaluated by NILM were recorded as positive TERC gain. The chi and Kruskal-Wallis 
      tests were used to assess the associations between FISH findings and diagnoses. 
      The incidence of positive TERC gain in the cases diagnosed as NILM or CIN1 was 
      significantly lower than that in cases diagnosed as CIN2, CIN3, or SCA (P < 
      0.01). In addition, a significantly higher proportion of abnormal cells with TERC 
      gain was found as a pathological change from CIN1 to SCA (P < 0.01). We conclude 
      that the TERC gain seems to be an important associated genetic event in CIN and 
      carcinoma; FISH is a potential tool for the diagnoses of uterine cervix disease.
FAU - Sui, Weiguo
AU  - Sui W
AD  - Laboratory Center of Guangzhou Military Area Command, 181st Hospital of People's 
      Liberation Army, Guilin, Guangxi, People's Republic of China.
FAU - Ou, Minglin
AU  - Ou M
FAU - Dai, Yong
AU  - Dai Y
FAU - Chen, Jiejing
AU  - Chen J
FAU - Lan, Huijuan
AU  - Lan H
FAU - Yan, Qiang
AU  - Yan Q
FAU - Huang, He
AU  - Huang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Gynecol Cancer
JT  - International journal of gynecological cancer : official journal of the 
      International Gynecological Cancer Society
JID - 9111626
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - Female
MH  - *Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Prognosis
MH  - RNA/*genetics
MH  - Telomerase/*genetics
MH  - Uterine Cervical Neoplasms/*genetics/pathology
MH  - Young Adult
MH  - Uterine Cervical Dysplasia/*genetics/pathology
EDAT- 2009/12/17 06:00
MHDA- 2010/03/03 06:00
CRDT- 2009/12/17 06:00
PHST- 2009/12/17 06:00 [entrez]
PHST- 2009/12/17 06:00 [pubmed]
PHST- 2010/03/03 06:00 [medline]
AID - 00009577-200911000-00003 [pii]
AID - 10.1111/IGC.0b013e3181b62ea5 [doi]
PST - ppublish
SO  - Int J Gynecol Cancer. 2009 Nov;19(8):1303-6. doi: 10.1111/IGC.0b013e3181b62ea5.