PMID- 20016034
OWN - NLM
STAT- MEDLINE
DCOM- 20100430
LR  - 20191210
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 91
IP  - Pt 5
DP  - 2010 May
TI  - RNA elements within the 5' untranslated region of the West Nile virus genome are 
      critical for RNA synthesis and virus replication.
PG  - 1218-23
LID - 10.1099/vir.0.013854-0 [doi]
AB  - RNA elements within the flavivirus genome may play essential regulatory roles 
      during virus replication. Here, recombinant West Nile virus (WNV) NS5 protein was 
      used in combination with WNV subgenomic RNA templates to establish in vitro 
      RNA-dependent RNA polymerase and RNA-binding assays. These assays identified 
      mutations in the stem-loop A (SLA) region of the 5' untranslated region (5'UTR) 
      altering NS5 RNA synthesis and RNA-binding capability. These mutations were then 
      introduced into the full-length WNV genome by reverse genetics. Further analysis 
      of the mutant viruses showed that deletion of nt 46-60, which disrupted the stem 
      and side loop of SLA, greatly compromised virus replication, whereas mutations 
      that destroyed the top loop of SLA required for RNA synthesis in vitro did not 
      significantly alter virus replication. These results suggest that SLA present in 
      the 5'UTR of WNV is essential for RNA synthesis in vitro and for virus 
      replication.
FAU - Li, Xiao-Feng
AU  - Li XF
AD  - State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, Beijing 100071, PR China.
FAU - Jiang, Tao
AU  - Jiang T
FAU - Yu, Xue-Dong
AU  - Yu XD
FAU - Deng, Yong-Qiang
AU  - Deng YQ
FAU - Zhao, Hui
AU  - Zhao H
FAU - Zhu, Qing-Yu
AU  - Zhu QY
FAU - Qin, E-De
AU  - Qin ED
FAU - Qin, Cheng-Feng
AU  - Qin CF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091216
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (5' Untranslated Regions)
RN  - 0 (NS5 protein, flavivirus)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Animals
MH  - Chlorocebus aethiops
MH  - Nucleic Acid Conformation
MH  - Protein Binding
MH  - RNA, Viral/*genetics/*metabolism
MH  - Sequence Deletion
MH  - Vero Cells
MH  - Viral Nonstructural Proteins/*metabolism
MH  - Viral Plaque Assay
MH  - *Virus Replication
MH  - West Nile virus/genetics/*physiology
EDAT- 2009/12/18 06:00
MHDA- 2010/05/01 06:00
CRDT- 2009/12/18 06:00
PHST- 2009/12/18 06:00 [entrez]
PHST- 2009/12/18 06:00 [pubmed]
PHST- 2010/05/01 06:00 [medline]
AID - vir.0.013854-0 [pii]
AID - 10.1099/vir.0.013854-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2010 May;91(Pt 5):1218-23. doi: 10.1099/vir.0.013854-0. Epub 2009 
      Dec 16.