PMID- 20016104
OWN - NLM
STAT- MEDLINE
DCOM- 20100126
LR  - 20211020
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 29
IP  - 50
DP  - 2009 Dec 16
TI  - Activity-dependent vesicular monoamine transporter-mediated depletion of the 
      nucleus supports somatic release by serotonin neurons.
PG  - 15878-87
LID - 10.1523/JNEUROSCI.4210-09.2009 [doi]
AB  - Packaging by the vesicular monoamine transporter (VMAT) is essential for 
      mood-controlling serotonin transmission but has not been assayed during activity. 
      Here, two-photon imaging of the fluorescent serotonin analog 
      5,7-dihydroxytryptamine and three-photon imaging of endogenous serotonin were 
      used to study vesicular packaging as it supports release from the soma of 
      serotonin neurons. Glutamate receptor activation in dorsal raphe brain slice 
      evoked somatic release that was mediated solely by vesicle exocytosis. This 
      release was accompanied by VMAT-mediated serotonin depletion from the nucleus, a 
      large compartment free of monoaminergic degradation pathways that has not been 
      implicated in neurotransmission previously. Finally, while some monoamine 
      packaged at rest was held in reserve, monoamine packaged during stimulation was 
      released completely. Hence, somatic vesicles loaded by VMAT during activity 
      rapidly undergo exocytosis. In the absence of active zones and with limited 
      neurotransmitter reuptake, somatic release by serotonin neurons is supported by 
      recruitment from a large pool of extravesicular serotonin in the nucleus and 
      cytoplasm, and preferential release of the newly packaged transmitter.
FAU - Colgan, Lesley A
AU  - Colgan LA
AD  - Department of Pharmacology and Chemical Biology, University of Pittsburgh, 
      Pittsburgh, Pennsylvania 15261, USA.
FAU - Putzier, Ilva
AU  - Putzier I
FAU - Levitan, Edwin S
AU  - Levitan ES
LA  - eng
GR  - R21DA25739/DA/NIDA NIH HHS/United States
GR  - R01 NS053050/NS/NINDS NIH HHS/United States
GR  - R01NS53050/NS/NINDS NIH HHS/United States
GR  - R01 NS053050-04/NS/NINDS NIH HHS/United States
GR  - R21 DA025739/DA/NIDA NIH HHS/United States
GR  - R21 DA025739-02/DA/NIDA NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Vesicular Monoamine Transport Proteins)
RN  - 31363-74-3 (5,7-Dihydroxytryptamine)
RN  - 333DO1RDJY (Serotonin)
SB  - IM
MH  - 5,7-Dihydroxytryptamine/pharmacology
MH  - Animals
MH  - Cell Nucleus/drug effects/*metabolism
MH  - Cells, Cultured
MH  - Exocytosis/drug effects/physiology
MH  - Male
MH  - Neurons/cytology/drug effects/*metabolism
MH  - PC12 Cells
MH  - Raphe Nuclei/cytology/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serotonin/*metabolism
MH  - Vesicular Monoamine Transport Proteins/*physiology
PMC - PMC2796554
MID - NIHMS158627
OTO - NOTNLM
OT  - SERT
OT  - VMAT2
OT  - fluorescent false neurotransmitter
OT  - multiphoton microscopy
OT  - somatodendritic release
OT  - vesicular transport
EDAT- 2009/12/18 06:00
MHDA- 2010/01/27 06:00
PMCR- 2010/06/16
CRDT- 2009/12/18 06:00
PHST- 2009/12/18 06:00 [entrez]
PHST- 2009/12/18 06:00 [pubmed]
PHST- 2010/01/27 06:00 [medline]
PHST- 2010/06/16 00:00 [pmc-release]
AID - 29/50/15878 [pii]
AID - 3554640 [pii]
AID - 10.1523/JNEUROSCI.4210-09.2009 [doi]
PST - ppublish
SO  - J Neurosci. 2009 Dec 16;29(50):15878-87. doi: 10.1523/JNEUROSCI.4210-09.2009.